Combination of procedure for ultra rapid extraction (PURE) and loop-mediated isothermal amplification (LAMP) for rapid detection of Mycoplasma bovis in milk.
Animals
Cattle
Colostrum
/ microbiology
DNA, Bacterial
/ isolation & purification
Female
Food Microbiology
/ methods
Mastitis, Bovine
/ diagnosis
Milk
/ microbiology
Molecular Diagnostic Techniques
/ methods
Mycoplasma Infections
/ diagnosis
Mycoplasma bovis
/ genetics
Nucleic Acid Amplification Techniques
/ methods
Polymerase Chain Reaction
/ veterinary
Sensitivity and Specificity
Mycoplasma bovis
loop-mediated isothermal amplification (LAMP)
milk infection
procedure for ultra rapid extraction (PURE)
rapid detection
Journal
The Journal of veterinary medical science
ISSN: 1347-7439
Titre abrégé: J Vet Med Sci
Pays: Japan
ID NLM: 9105360
Informations de publication
Date de publication:
10 Jul 2020
10 Jul 2020
Historique:
pubmed:
26
5
2020
medline:
6
3
2021
entrez:
26
5
2020
Statut:
ppublish
Résumé
Polymerase chain reaction (PCR) is typically used for the early detection of mycoplasma in bovine milk; it requires 3 days to obtain results because of the necessary enrichment process. A more rapid, simple, and accurate detection method is required to directly detect the Mycoplasma bovis (M. bovis) gene in milk. In this study, we assess the utility of combining the following two methods to achieve this goal: the loop-mediated isothermal amplification (LAMP), which is more sensitive than PCR, and the procedure for ultra rapid extraction (PURE), which adsorbs and filters components that inhibit DNA amplification/detection. LAMP was examined using DNA extracts obtained by four methods. This showed that PURE had the highest sensitivity and specificity and that the combination of PURE and LAMP was able to detect M. bovis in milk. We then showed that the detection limit of M. bovis was 10
Identifiants
pubmed: 32448815
doi: 10.1292/jvms.19-0695
pmc: PMC7399326
doi:
Substances chimiques
DNA, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
875-880Références
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