Generation of twenty four induced pluripotent stem cell lines from twenty four members of the Lothian Birth Cohort 1936.

Cell Differentiation Cellular Reprogramming Induced Pluripotent Stem Cells Karyotype Leukocytes, Mononuclear Plasmids

Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
07 2020
Historique:
received: 14 01 2020
revised: 01 05 2020
accepted: 13 05 2020
pubmed: 26 5 2020
medline: 22 6 2021
entrez: 26 5 2020
Statut: ppublish

Résumé

Cognitive decline is among the most feared aspects of ageing. We have generated induced pluripotent stem cells (iPSCs) from 24 people from the Lothian Birth Cohort 1936, whose cognitive ability was tested in childhood and in older age. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using non-integrating oriP/EBNA1 backbone plasmids expressing six iPSC reprogramming factors (OCT3/4 (POU5F1), SOX2, KLF4, L-Myc, shp53, Lin28, SV40LT). All lines demonstrated STR matched karyotype and pluripotency was validated by multiple methods. These iPSC lines are a valuable resource to study molecular mechanisms underlying individual differences in cognitive ageing and resilience to age-related neurodegenerative diseases.

Identifiants

DOI: 10.1016/j.scr.2020.101851 PMID: 32450543 PMC: PMC7347008
pubmed: 32450543
pii: S1873-5061(20)30152-5
doi: 10.1016/j.scr.2020.101851
pmc: PMC7347008
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101851

Subventions

Organisme : Medical Research Council
ID : MR/K026992/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M013111/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/R024065/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/L023784/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G0700704
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_EX_MR/N50192X/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N013255/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/M024962/1
Pays : United Kingdom

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: US patent US 10,221,395 B2 has been granted describing some of the methods to reprogram to iPSCs. Apart from this issued patent filing the authors have declared that no other competing financial interests exist.

Références

Mutat Res. 2001 Nov 15;498(1-2):159-67
pubmed: 11673081
Int J Stroke. 2011 Dec;6(6):547-59
pubmed: 22111801
Int J Epidemiol. 2018 Aug 1;47(4):1042-1042r
pubmed: 29546429

Auteurs

Jamie Toombs (J)

Centre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, UK.

Lindsay Panther (L)

iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.

Loren Ornelas (L)

iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.

Chunyan Liu (C)

iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.

Emilda Gomez (E)

iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.

Raquel Martín-Ibáñez (R)

Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA.

Simon R Cox (SR)

Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.

Stuart J Ritchie (SJ)

Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.

Sarah E Harris (SE)

Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.

Adele Taylor (A)

Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.

Paul Redmond (P)

Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.

Tom C Russ (TC)

Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK; Alzheimer Scotland Dementia Research Centre, University of Edinburgh, Edinburgh, UK.

Lee Murphy (L)

Edinburgh Clinical Research Facility, University of Edinburgh, Edinburgh, UK.

James D Cooper (JD)

Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK.

Karen Burr (K)

Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK.

Bhuvaneish T Selvaraj (BT)

Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK.

Cathy Browne (C)

James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK.

Clive N Svendsen (CN)

Board of Governors-Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA.

Sally A Cowley (SA)

James Martin Stem Cell Facility, Sir William Dunn School of Pathology, University of Oxford, Oxford, UK; Oxford Parkinson's Disease Centre, Oxford, UK.

Ian J Deary (IJ)

Lothian Birth Cohorts, Department of Psychology, University of Edinburgh, Edinburgh, UK.

Siddharthan Chandran (S)

Dementia Research Institute at the University of Edinburgh, UK; Centre for Clinical Brain Sciences, University of Edinburgh, Edinburgh, Scotland, UK.

Tara L Spires-Jones (TL)

Centre for Discovery Brain Sciences, UK Dementia Research Institute, The University of Edinburgh, UK. Electronic address: tara.spires-jones@ed.ac.uk.

Dhruv Sareen (D)

iPSC Core, The David Janet Polak Foundation Stem Cell Core Laboratory, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Cedars-Sinai Biomanufacturing Center, West Hollywood, CA 90069, USA; Board of Governors-Regenerative Medicine Institute, Cedars-Sinai Medical Center, Los Angeles, CA 90048, USA; Department of Biomedical Sciences, Cedars-Sinai Medical Center, Los Angeles, CA, 90048, USA. Electronic address: dhruv.sareen@cshs.org.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Différenciation cellulaire › Generation of twenty four induced pluripotent...
questionsmedicales.fr › Phénomènes génétiques › Reprogrammation cellulaire › Generation of twenty four induced pluripotent...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches pluripotentes › Cellules souches pluripotentes induites › Generation of twenty four induced pluripotent...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Chromosomes › Caryotype › Generation of twenty four induced pluripotent...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Generation of twenty four induced pluripotent...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › Generation of twenty four induced pluripotent...