Cell Density-Dependent Fibroblast Growth Factor-2 Signaling Regulates Syndecan-4 Expression in Cultured Vascular Endothelial Cells.
FGF-2
cell density
endothelial cell
proteoglycan
syndecan-4
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
24 May 2020
24 May 2020
Historique:
received:
12
03
2020
revised:
14
05
2020
accepted:
22
05
2020
entrez:
28
5
2020
pubmed:
28
5
2020
medline:
17
2
2021
Statut:
epublish
Résumé
Syndecan-4 is a member of the syndecan family of transmembrane heparan sulfate proteoglycans, and is involved in cell protection, proliferation, and the blood coagulation-fibrinolytic system in vascular endothelial cells. Heparan sulfate chains enable fibroblast growth factor-2 (FGF-2) to form a complex with its receptor and to transduce the cell growth signal. In the present study, bovine aortic endothelial cells were cultured, and the intracellular signal pathways that mediate the regulation of syndecan-4 expression in dense and sparse cultures by FGF-2 were analyzed. We demonstrated the cell density-dependent differential regulation of syndecan-4 expression. Specifically, we found that FGF-2 upregulated the synthesis of syndecan-4 in vascular endothelial cells via the MEK1/2-ERK1/2 pathway in dense cell cultures, with only a transcriptional induction of syndecan-4 at a low cell density via the Akt pathway. This study highlights a critical mechanism underlying the regulation of endothelial cell functions by proteoglycans.
Identifiants
pubmed: 32456321
pii: ijms21103698
doi: 10.3390/ijms21103698
pmc: PMC7279341
pii:
doi:
Substances chimiques
Syndecan-4
0
Fibroblast Growth Factor 2
103107-01-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : JP 19K19418
Organisme : Japan Society for the Promotion of Science
ID : JP 18K06638
Organisme : Japan Society for the Promotion of Science
ID : JP 19K07089
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