Single cell transcriptomics reveals opioid usage evokes widespread suppression of antiviral gene program.
Adult
Antiviral Agents
/ pharmacology
Female
Gene Expression Profiling
Gene Expression Regulation
/ drug effects
Humans
Immunity, Innate
/ genetics
Interferons
/ pharmacology
Leukocytes, Mononuclear
Lipopolysaccharides
/ pharmacology
Male
Middle Aged
Morphine
/ pharmacology
Opioid-Related Disorders
/ immunology
Single-Cell Analysis
Virus Diseases
/ immunology
Young Adult
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 05 2020
26 05 2020
Historique:
received:
18
07
2019
accepted:
19
04
2020
entrez:
28
5
2020
pubmed:
28
5
2020
medline:
19
8
2020
Statut:
epublish
Résumé
Chronic opioid usage not only causes addiction behavior through the central nervous system, but also modulates the peripheral immune system. However, how opioid impacts the immune system is still barely characterized systematically. In order to understand the immune modulatory effect of opioids in an unbiased way, here we perform single-cell RNA sequencing (scRNA-seq) of peripheral blood mononuclear cells from opioid-dependent individuals and controls to show that chronic opioid usage evokes widespread suppression of antiviral gene program in naive monocytes, as well as in multiple immune cell types upon stimulation with the pathogen component lipopolysaccharide. Furthermore, scRNA-seq reveals the same phenomenon after a short in vitro morphine treatment. These findings indicate that both acute and chronic opioid exposure may be harmful to our immune system by suppressing the antiviral gene program. Our results suggest that further characterization of the immune modulatory effects of opioid is critical to ensure the safety of clinical opioids.
Identifiants
pubmed: 32457298
doi: 10.1038/s41467-020-16159-y
pii: 10.1038/s41467-020-16159-y
pmc: PMC7250875
doi:
Substances chimiques
Antiviral Agents
0
Lipopolysaccharides
0
Morphine
76I7G6D29C
Interferons
9008-11-1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2611Subventions
Organisme : NIDA NIH HHS
ID : R01 DA046436
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI138960
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM100842
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI042853
Pays : United States
Organisme : NIDA NIH HHS
ID : R33 DA047032
Pays : United States
Organisme : NIDA NIH HHS
ID : R61 DA047032
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA017305
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA042620
Pays : United States
Organisme : NIDA NIH HHS
ID : R33 DA041883
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA051889
Pays : United States
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