Evolutionary analyses reveal independent origins of gene repertoires and structural motifs associated to fast inactivation in calcium-selective TRPV channels.
Amino Acid Sequence
Amphibians
/ metabolism
Animals
Birds
/ metabolism
Calcium
/ metabolism
Evolution, Molecular
Gills
/ metabolism
HEK293 Cells
Helix-Loop-Helix Motifs
Humans
Kidney
/ metabolism
Mammals
/ metabolism
Mutagenesis, Site-Directed
Phylogeny
Sequence Alignment
TRPV Cation Channels
/ chemistry
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
26 05 2020
26 05 2020
Historique:
received:
21
01
2020
accepted:
23
04
2020
entrez:
28
5
2020
pubmed:
28
5
2020
medline:
28
11
2020
Statut:
epublish
Résumé
Essential for calcium homeostasis, TRPV5 and TRPV6 are calcium-selective channels belonging to the transient receptor potential (TRP) gene family. In this study, we investigated the evolutionary history of these channels to add an evolutionary context to the already available physiological information. Phylogenetic analyses revealed that paralogs found in mammals, sauropsids, amphibians, and chondrichthyes, are the product of independent duplication events in the ancestor of each group. Within amniotes, we identified a traceable signature of three amino acids located at the amino-terminal intracellular region. The signature correlates with both the duplication events and the phenotype of fast inactivation observed in mammalian TRPV6 channels. Electrophysiological recordings and mutagenesis revealed that the signature sequence modulates the phenotype of fast inactivation in all clades of vertebrates but reptiles. A transcriptome analysis showed a change in tissue expression from gills, in marine vertebrates, to kidneys in terrestrial vertebrates. Our results highlight a cytoplasmatic structural triad composed by the Helix-Loop-Helix domain, the S2-S3 linker, and the TRP domain helix that is important on modulating the activity of calcium-selective TRPV channels.
Identifiants
pubmed: 32457384
doi: 10.1038/s41598-020-65679-6
pii: 10.1038/s41598-020-65679-6
pmc: PMC7250927
doi:
Substances chimiques
TRPV Cation Channels
0
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
8684Références
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