Cell-free protein synthesis in hydrogel materials.

Biocompatible Materials / chemistry Cell Extracts Cell Line DNA / metabolism Hydrogels / chemistry Polyethylene Glycols / chemistry Polymers / chemistry Protein Biosynthesis / genetics Proteins / genetics Sepharose / chemistry Tissue Scaffolds / chemistry

Journal

Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838

Informations de publication

Date de publication:
04 Jul 2020
Historique:
pubmed: 28 5 2020
medline: 4 2 2021
entrez: 28 5 2020
Statut: ppublish

Résumé

We report a method for embedding cell-free protein synthesis reactions in macro-scale hydrogel materials without a free liquid phase. This paper focuses on methods of preparation for a variety of hydrogels and an investigation of the impact that the hydrogel material has on cell-free protein synthesis.

Identifiants

DOI: 10.1039/d0cc02582h PMID: 32458833
pubmed: 32458833
doi: 10.1039/d0cc02582h
doi:

Substances chimiques

Biocompatible Materials 0
Cell Extracts 0
Hydrogels 0
Polymers 0
Proteins 0
Polyethylene Glycols 3WJQ0SDW1A
DNA 9007-49-2
Sepharose 9012-36-6

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

7108-7111

Auteurs

Colette J Whitfield (CJ)

School of Natural and Environmental Sciences, Faculty of Science, Agriculture and Engineering, Newcastle University, Newcastle, UK. thomas.howard@newcastle.ac.uk.

Alice M Banks (AM)

Gema Dura (G)

John Love (J)

Jonathan E Fieldsend (JE)

Sarah A Goodchild (SA)

David A Fulton (DA)

Thomas P Howard (TP)

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Matériaux biocompatibles › Cell-free protein synthesis in hydrogel materials.
questionsmedicales.fr › Mélanges complexes › Extraits tissulaires › Extrait cellulaire › Cell-free protein synthesis in hydrogel materials.
questionsmedicales.fr › Cellules › Cellules cultivées › Lignée cellulaire › Cell-free protein synthesis in hydrogel materials.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › Cell-free protein synthesis in hydrogel materials.
questionsmedicales.fr › Préparations pharmaceutiques › Colloïdes › Gels › Hydrogels › Cell-free protein synthesis in hydrogel materials.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Polyéthylène glycols › Cell-free protein synthesis in hydrogel materials.
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Cell-free protein synthesis in hydrogel materials.
questionsmedicales.fr › Phénomènes génétiques › Expression des gènes › Biosynthèse des protéines › Cell-free protein synthesis in hydrogel materials.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Cell-free protein synthesis in hydrogel materials.
questionsmedicales.fr › Glucides › Polyosides › Agarose › Cell-free protein synthesis in hydrogel materials.
questionsmedicales.fr › Équipement et fournitures › Prothèses et implants › Structures d'échafaudage tissulaires › Cell-free protein synthesis in hydrogel materials.