Generation of three induced pluripotent stem cell (iPSC) lines from a multiple endocrine neoplasia type 1 (MEN1) patient and three iPSC lines from an unaffected relative of the patient.


Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
07 2020
Historique:
received: 18 02 2020
revised: 20 04 2020
accepted: 11 05 2020
pubmed: 28 5 2020
medline: 22 6 2021
entrez: 28 5 2020
Statut: ppublish

Résumé

We generated three disease-specific iPSC lines from a Multiple endocrine neoplasia type 1 (MEN1) patient and three control iPSC lines from an unaffected blood relative of the patient using unutilized lymphoblastoid B cell lines (LCLs) as a cell resource. The expression of pluripotency markers, retaining of normal karyotype of chromosome, absence of episomal vectors used for generating the iPSCs and EBV used for generating LCLs, and the potential to differentiate into three germ layers, were confirmed for each iPSC line. These iPSC lines can be useful for construction of the disease models in vitro, and elucidation of the disease mechanisms.

Identifiants

pubmed: 32460232
pii: S1873-5061(20)30147-1
doi: 10.1016/j.scr.2020.101846
pii:
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101846

Informations de copyright

Copyright © 2020. Published by Elsevier B.V.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Satoru Yoshida (S)

Department of Regenerative Medicine and Stem Cell Biology, Fujita Health University School of Medicine, Toyoake, Japan.

Hanayuki Okura (H)

Department of Regenerative Medicine Support Promotion Facility, Center for Research Promotion and Support, Fujita Health University, Toyoake, Japan.

Hidetaka Suga (H)

Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Tomohiko Nishitomi (T)

Department of Regenerative Medicine and Stem Cell Biology, Fujita Health University School of Medicine, Toyoake, Japan.

Akihiro Sakurai (A)

Department of Medical Genetics and Genomics, Sapporo Medical University School of Medicine, Sapporo, Japan.

Hiroshi Arima (H)

Department of Endocrinology and Diabetes, Nagoya University Graduate School of Medicine, Nagoya, Japan.

Akifumi Matsuyama (A)

Department of Regenerative Medicine and Stem Cell Biology, Fujita Health University School of Medicine, Toyoake, Japan. Electronic address: akifumi-matsuyama@umin.ac.jp.

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Classifications MeSH