Dynamical in-situ observation of the lyophilization and vacuum-drying processes of a model biopharmaceutical system by an environmental scanning electron microscope.


Journal

International journal of pharmaceutics
ISSN: 1873-3476
Titre abrégé: Int J Pharm
Pays: Netherlands
ID NLM: 7804127

Informations de publication

Date de publication:
30 Jul 2020
Historique:
received: 16 12 2019
revised: 23 04 2020
accepted: 18 05 2020
pubmed: 29 5 2020
medline: 4 3 2021
entrez: 29 5 2020
Statut: ppublish

Résumé

The paper discusses the real-time monitoring of the changing sample morphology during the entire lyophilization (freeze-drying) and vacuum-drying processes of model biopharmaceutical solutions by using an environmental scanning electron microscope (ESEM); the device's micromanipulators were used to study the interior of the samples in-situ without exposing the samples to atmospheric water vapor. The individual collapse temperatures (T

Identifiants

pubmed: 32461002
pii: S0378-5173(20)30432-4
doi: 10.1016/j.ijpharm.2020.119448
pii:
doi:

Substances chimiques

Serum Albumin, Bovine 27432CM55Q
Sucrose 57-50-1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

119448

Informations de copyright

Copyright © 2020 Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Ľubica Vetráková (Ľ)

Environmental Electron Microscopy Group, Institute of Scientific Instruments of the Czech Academy of Sciences, Brno, Czech Republic. Electronic address: vetrakova@isibrno.cz.

Vilém Neděla (V)

Environmental Electron Microscopy Group, Institute of Scientific Instruments of the Czech Academy of Sciences, Brno, Czech Republic.

Jiří Runštuk (J)

Environmental Electron Microscopy Group, Institute of Scientific Instruments of the Czech Academy of Sciences, Brno, Czech Republic.

Eva Tihlaříková (E)

Environmental Electron Microscopy Group, Institute of Scientific Instruments of the Czech Academy of Sciences, Brno, Czech Republic.

Dominik Heger (D)

Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic. Electronic address: hegerd@chemi.muni.cz.

Evgenyi Shalaev (E)

Pharmaceutical Development, Allergan plc, Irvine, CA, United States. Electronic address: evgenyi.shalaev@allergan.com.

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Classifications MeSH