Antibiotic susceptibility testing of anaerobic bacteria by broth microdilution method using the MICRONAUT-S Anaerobes MIC plates.

Anti-Bacterial Agents / pharmacology Bacteria, Anaerobic / drug effects Microbial Sensitivity Tests / methods
Anaerobes Antimicrobial susceptibility testing Broth microdilution method MICRONAUT-S Anaerobes MIC Test plate

Journal

Anaerobe
ISSN: 1095-8274
Titre abrégé: Anaerobe
Pays: England
ID NLM: 9505216

Informations de publication

Date de publication:
Jun 2020
Historique:
received: 04 03 2020
revised: 20 05 2020
accepted: 21 05 2020
pubmed: 29 5 2020
medline: 12 1 2021
entrez: 29 5 2020
Statut: ppublish

Résumé

Susceptibility profiles of anaerobic bacteria to antibiotics have become unpredictable, thus reliable and user-friendly methods for routine susceptibility testing are needed. In this study, we evaluated the MICRONAUT-S Anaerobes MIC test plate, a commercially available broth microdilution method, and suitable for clinical microbiology routine testing. We analyzed a collection of 300 consecutive clinically significant isolates, including 149 Gram-positive and 151 Gram-negative strains. The performance of the MICRONAUT-S Anaerobes MIC plate was compared to that of a gradient diffusion method (current laboratory standard), calculating the essential and the categorial agreement. 99.7% (299/300) of the strains included in this study successfully grew in the MICRONAUT-S Anaerobes MIC plate (73% of them after 24 h of incubation), while 1 Porphyromonas uenoni isolate didn't grow. It showed a high concordance with the gradient diffusion method. Overall essential and categorical agreements resulted >95% and >97%, respectively, and only a low rate of errors was observed. Beyond the very good analytical performance, several technical advantages in comparison with the gradient diffusion method were observed, that contribute to make the MICRONAUT-S Anaerobes panels suitable for an easy implementation into laboratory routine.

Identifiants

DOI: 10.1016/j.anaerobe.2020.102217 PMID: 32461082
pubmed: 32461082
pii: S1075-9964(20)30073-1
doi: 10.1016/j.anaerobe.2020.102217
pii:
doi:

Substances chimiques

Anti-Bacterial Agents 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102217

Informations de copyright

Copyright © 2020 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest None.

Auteurs

Miriam Cordovana (M)

University Hospital of Bologna-Policlinico Sant'Orsola-Malpighi, Operative Unit of Microbiology, via Massarenti, 9, Bologna, Italy. Electronic address: miri-78@live.it.

Simone Ambretti (S)

University Hospital of Bologna-Policlinico Sant'Orsola-Malpighi, Operative Unit of Microbiology, via Massarenti, 9, Bologna, Italy.

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Utilisations thérapeutiques › Anti-infectieux › Antibactériens › Antibiotic susceptibility testing of anaerobic...
questionsmedicales.fr › Bactéries › Bactéries anaérobies › Antibiotic susceptibility testing of anaerobic...
questionsmedicales.fr › Techniques d'investigation › Évaluation préclinique de médicament › Tests de sensibilité microbienne › Antibiotic susceptibility testing of anaerobic...