Molecular characterization of hepatitis A virus circulating in Uttar Pradesh, India: A hospital-based study.
AVH
HAV
India
endemic
genotype
hepatitis A
Journal
The Indian journal of medical research
ISSN: 0971-5916
Titre abrégé: Indian J Med Res
Pays: India
ID NLM: 0374701
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
entrez:
29
5
2020
pubmed:
29
5
2020
medline:
4
3
2021
Statut:
ppublish
Résumé
Hepatitis A is prevalent worldwide and is among the leading cause of acute viral hepatitis in India. Major geographical differences in endemicity of hepatitis A are closely related to hygienic and sanitary conditions and other indicators of the level of socio-economic development. The present study was aimed to know the seropositivity prevalence and predominant circulating strain of HAV in a north India. Patients with acute viral hepatitis were enrolled. Blood samples were collected over a period of one year from June 2016 to May 2017. Serum samples were tested for anti-immunoglobulin M (IgM) HAV antibodies. The seropositive samples were analyzed for HAV-RNA by real-time reverse transcription-polymerase chain reaction (RT-PCR). Samples detected on molecular assay were subjected to conventional semi-nested RT-PCR for VP1 gene. Further sequencing of amplified RT-PCR products was done, and data were analyzed. A total of 1615 patients were enrolled, and serum samples were collected and tested. The male:female ratio was 1.3:1 with a mean age of 24.31±17.02 yr (range 0-83 yr). Among these, 128 (7.93%) were positive for anti-HAV IgM antibodies; 41.63 per cent of seropositive patients were in their childhood or early adolescent age group. Of all seropositive samples, 59 (46.09%) were positive for HAV RNA. Genotyping sequencing of 10 representative strains was carried out, and the circulating genotype was found to be IIIA. The nucleotide sequences showed homology among the strains. Our results showed that hepatitis A was a common disease in children with IIIA as a circulating genotype in this region. In approximately 50 per cent of cases, HAV RNA could be detected. Higher number of HAV IgM-seropositive cases was observed during monsoon period.
Sections du résumé
Background & objectives
Hepatitis A is prevalent worldwide and is among the leading cause of acute viral hepatitis in India. Major geographical differences in endemicity of hepatitis A are closely related to hygienic and sanitary conditions and other indicators of the level of socio-economic development. The present study was aimed to know the seropositivity prevalence and predominant circulating strain of HAV in a north India.
Methods
Patients with acute viral hepatitis were enrolled. Blood samples were collected over a period of one year from June 2016 to May 2017. Serum samples were tested for anti-immunoglobulin M (IgM) HAV antibodies. The seropositive samples were analyzed for HAV-RNA by real-time reverse transcription-polymerase chain reaction (RT-PCR). Samples detected on molecular assay were subjected to conventional semi-nested RT-PCR for VP1 gene. Further sequencing of amplified RT-PCR products was done, and data were analyzed.
Results
A total of 1615 patients were enrolled, and serum samples were collected and tested. The male:female ratio was 1.3:1 with a mean age of 24.31±17.02 yr (range 0-83 yr). Among these, 128 (7.93%) were positive for anti-HAV IgM antibodies; 41.63 per cent of seropositive patients were in their childhood or early adolescent age group. Of all seropositive samples, 59 (46.09%) were positive for HAV RNA. Genotyping sequencing of 10 representative strains was carried out, and the circulating genotype was found to be IIIA. The nucleotide sequences showed homology among the strains.
Interpretation & conclusions
Our results showed that hepatitis A was a common disease in children with IIIA as a circulating genotype in this region. In approximately 50 per cent of cases, HAV RNA could be detected. Higher number of HAV IgM-seropositive cases was observed during monsoon period.
Identifiants
pubmed: 32461402
pii: IndianJMedRes_2020_151_4_375_284217
doi: 10.4103/ijmr.IJMR_429_18
pmc: PMC7371058
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
375-379Déclaration de conflit d'intérêts
None
Références
Indian J Med Res. 2009 Aug;130(2):179-84
pubmed: 19797816
J Clin Microbiol. 2004 Jun;42(6):2825-8
pubmed: 15184483
Indian J Med Res. 2008 Dec;128(6):699-704
pubmed: 19246792
Indian J Med Res. 2015 Feb;141(2):213-20
pubmed: 25900957
Indian J Med Microbiol. 2013 Jul-Sep;31(3):261-5
pubmed: 23883712
J Gastroenterol Hepatol. 2006 Apr;21(4):689-93
pubmed: 16677154
J Gen Virol. 2003 Dec;84(Pt 12):3191-3201
pubmed: 14645901
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
Indian J Med Res. 2014 Jun;139(6):940-4
pubmed: 25109730
J Med Virol. 2003 Jun;70(2):187-93
pubmed: 12696104
Indian J Med Res. 1999 Jan;109:11-5
pubmed: 10489736
J Gen Virol. 1999 Mar;80 ( Pt 3):653-662
pubmed: 10092005
MMWR Surveill Summ. 2008 Mar 21;57(2):1-24
pubmed: 18354374
Indian J Med Res. 2006 Jun;123(6):760-9
pubmed: 16885597
Appl Environ Microbiol. 2006 Jun;72(6):3846-55
pubmed: 16751488
J Gen Virol. 1992 Jun;73 ( Pt 6):1365-77
pubmed: 1318940