Chemical composition analysis of pomegranate seeds based on ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap high-resolution mass spectrometry.
Chemical composition
Fragmentation pathway
Pomegranate seeds
Quadrupole-Orbitrap high-resolution mass spectrometry
Ultra-high-performance liquid chromatography
Ultrasonic/microwave-assisted extraction
Journal
Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336
Informations de publication
Date de publication:
05 Aug 2020
05 Aug 2020
Historique:
received:
17
02
2020
revised:
01
05
2020
accepted:
04
05
2020
pubmed:
29
5
2020
medline:
10
4
2021
entrez:
29
5
2020
Statut:
ppublish
Résumé
Pomegranate fruit has shown a variety of pharmacological effects, however, the bioactive constituents in pomegranate seeds have not been fully identified. To this end, the chemical compositions of pomegranate seeds were analyzed using ultra-high-performance liquid chromatography (UHPLC) coupled with quadrupole-Orbitrap high-resolution mass spectrometry (Q-Orbitrap HRMS). Prior to analysis, pomegranate seed samples were treated by ultrasonic/microwave-assisted extraction (UMAE). A total of 88 chemical compounds were tentatively identified based on accurate-mass measurements for precursor and fragment ions, including flavonoids, coumarins, phenolic acids, amino acids, and nucleosides. Their mass spectrometric fragmentation pathways were investigated. The study has revealed the nutritional value of pomegranate seeds, paving the way for future further applications of pomegranate seeds in nutrition and health care industry.
Identifiants
pubmed: 32464591
pii: S0731-7085(20)30398-8
doi: 10.1016/j.jpba.2020.113357
pii:
doi:
Substances chimiques
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
113357Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.