A simple, rapid typing method for Streptococcus agalactiae based on ribosomal subunit proteins by MALDI-TOF MS.
Animals
Bacterial Typing Techniques
/ methods
Fishes
/ microbiology
Humans
Molecular Weight
Phylogeny
Ribosome Subunits
/ metabolism
Serotyping
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Streptococcal Infections
/ microbiology
Streptococcus agalactiae
/ classification
Swine
/ microbiology
Zoonoses
/ microbiology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 05 2020
29 05 2020
Historique:
received:
09
02
2020
accepted:
24
04
2020
entrez:
31
5
2020
pubmed:
31
5
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Streptococcus agalactiae (Group B Streptococcus, GBS), is a frequent human colonizer and a leading cause of neonatal meningitis as well as an emerging pathogen in non-pregnant adults. GBS possesses a broad animal host spectrum, and recent studies proved atypical GBS genotypes can cause human invasive diseases through animal sources as food-borne zoonotic infections. We applied a MALDI-TOF MS typing method, based on molecular weight variations of predefined 28 ribosomal subunit proteins (rsp) to classify GBS strains of varying serotypes into major phylogenetic lineages. A total of 249 GBS isolates of representative and varying capsular serotypes from patients and animal food sources (fish and pig) collected during 2016-2018 in Hong Kong were analysed. Over 84% (143/171) noninvasive carriage GBS strains from patients were readily typed into 5 globally dominant rsp-profiles. Among GBS strains from food animals, over 90% (57/63) of fish and 13% (2/15) of pig GBS matched with existing rsp-profiles, while the remainder were classified into two novel rsp-profiles and we failed to assign a fish strain into any cluster. MALDI-TOF MS allowed for high-throughput screening and simultaneous detection of novel, so far not well described GBS genotypes. The method shown here is rapid, simple, readily transferable and adapted for use in a diagnostic microbiology laboratory with potential for the surveillance of emerging GBS genotypes with zoonotic potential.
Identifiants
pubmed: 32472028
doi: 10.1038/s41598-020-65707-5
pii: 10.1038/s41598-020-65707-5
pmc: PMC7260235
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8788Références
Farley, M. M. Group B Streptococcal Disease in Nonpregnant Adults. Clinical Infectious Diseases 33, 556–561 (2001).
doi: 10.1086/322696
Oliveira, I. et al. Genetic relatedness between group B streptococci originating from bovine mastitis and a human group B streptococcus type V cluster displaying an identical pulsed-field gel electrophoresis pattern. Clinical Microbiology and Infection 12, 887–893 (2006).
doi: 10.1111/j.1469-0691.2006.01508.x
Wibawan, I. W., Lämmler, C. & Smola, J. Properties and type antigen patterns of group B streptococcal isolates from pigs and nutrias. Journal of Clinical Microbiology 31, 762–764 (1993).
doi: 10.1128/JCM.31.3.762-764.1993
Rothen, J. et al. Draft Genome Sequences of Seven Streptococcus agalactiae strains isolated from camelus dromedarius at the horn of Africa. Genome Announcements 5, (2017).
Evans, J. J., Klesius, P. H., Pasnik, D. J. & Bohnsack, J. F. Human Streptococcus agalactiae Isolate in Nile Tilapia (Oreochromis niloticus). Emerging Infectious Diseases 15, 774–776 (2009).
doi: 10.3201/eid1505.080222
Delannoy, C. M. et al. Human Streptococcus agalactiae strains in aquatic mammals and fish. BMC Microbiology 13, 41 (2013).
doi: 10.1186/1471-2180-13-41
Madrid, L. et al. Infant Group B streptococcal disease incidence and serotypes worldwide: systematic review and meta-analyses. Clinical Infectious Diseases 65, (2017).
Ip, M. et al. Hypervirulent clone of group B Streptococcus serotype iii sequence type 283, Hong Kong, 1993–2012. Emerging Infectious Diseases 22, 1800–1803 (2016).
doi: 10.3201/eid2210.151436
Yang, Y. et al. Role of two-component system response regulator bcer in the antimicrobial resistance, virulence, biofilm formation, and stress response of group B Streptococcus. Frontiers in Microbiology 10, (2019).
Shabayek, S. & Spellerberg, B. Group B streptococcal colonization, molecular characteristics, and epidemiology. Frontiers in Microbiology 9, (2018).
Tan, S. et al. Group B Streptococcus serotype III sequence type 283 bacteremia associated with consumption of raw fish, Singapore. Emerging Infectious Diseases 22, 1970–1973 (2016).
doi: 10.3201/eid2211.160210
Ip, M. et al. Identification of a Streptococcus agalactiae Serotype III Subtype 4 clone in association with adult invasive disease in Hong Kong. Journal of Clinical Microbiology 44, 4252–4254 (2006).
doi: 10.1128/JCM.01533-06
Kalimuddin, S. et al. 2015 Epidemic of severe streptococcus agalactiae sequence type 283 infections in Singapore associated with the consumption of raw freshwater fish: a detailed analysis of clinical, epidemiological, and bacterial sequencing data. Clinical Infectious Diseases 64, (2017).
doi: 10.1093/cid/cix021
Seng, P. et al. Ongoing revolution in bacteriology: routine identification of bacteria by matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry. Clinical Infectious Diseases 49, 543–551 (2009).
doi: 10.1086/600885
Singhal, N., Kumar, M., Kanaujia, P. K. & Virdi, J. S. MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis. Frontiers in Microbiology 6, (2015).
Binghuai, L. et al. Use of MALDI-TOF mass spectrometry for rapid identification of group B Streptococcus on chromID Strepto B agar. International Journal of Infectious Diseases 27, 44–48 (2014).
doi: 10.1016/j.ijid.2014.06.023
Cherkaoui, A., Emonet, S., Fernandez, J., Schorderet, D. & Schrenzel, J. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification of beta-hemolytic streptococci. Journal of Clinical Microbiology 49, 3004–3005 (2011).
doi: 10.1128/JCM.00240-11
Lartigue, M.-F. et al. Identification of Streptococcus agalactiae isolates from various phylogenetic lineages by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Journal of Clinical Microbiology 47, 2284–2287 (2009).
doi: 10.1128/JCM.00175-09
Lartigue, M.-F. et al. Rapid detection of “highly virulent” group B Streptococcus ST-17 and emerging ST-1 clones by MALDI-TOF mass spectrometry. Journal of Microbiological Methods 86, 262–265 (2011).
doi: 10.1016/j.mimet.2011.05.017
Rothen, J. et al. Subspecies typing of Streptococcus agalactiae based on ribosomal subunit protein mass variation by MALDI-TOF MS. Frontiers in Microbiology 10, (2019).
Imperi, M. et al. A multiplex PCR assay for the direct identification of the capsular type (Ia to IX) of Streptococcus agalactiae. Journal of Microbiological Methods 80, 212–214 (2010).
doi: 10.1016/j.mimet.2009.11.010
Gibb, S. & Strimmer, K. MALDIquant: a versatile R package for the analysis of mass spectrometry data. Bioinformatics 28, 2270–2271 (2012).
doi: 10.1093/bioinformatics/bts447
Arnold, R. J. & Reilly, J. P. Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry. Analytical Biochemistry 269, 105–112 (1999).
doi: 10.1006/abio.1998.3077
Konstantinidis, K. T. & Tiedje, J. M. Genomic insights that advance the species definition for prokaryotes. Proceedings of the National Academy of Sciences 102, 2567–2572 (2005).
doi: 10.1073/pnas.0409727102
Letunic, I. & Bork, P. Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees. Nucleic Acids Research 44, (2016).
Jolley, K. A. & Maiden, M. C. BIGSdb: Scalable analysis of bacterial genome variation at the population level. BMC Bioinformatics 11, (2010).
Lin, H.-C. et al. Identification of a proteomic biomarker associated with invasive ST1, serotype VI group B Streptococcus by MALDI-TOF MS. Journal of Microbiology, Immunology and Infection 52, 81–89 (2019).
doi: 10.1016/j.jmii.2017.11.007