Disposable immunoplatforms for the simultaneous determination of biomarkers for neurodegenerative disorders using poly(amidoamine) dendrimer/gold nanoparticle nanocomposite.
Amperometric detection
Brain tissue extracts
Dual immunosensing platform
Plasma
TDP-43
Tau
Journal
Analytical and bioanalytical chemistry
ISSN: 1618-2650
Titre abrégé: Anal Bioanal Chem
Pays: Germany
ID NLM: 101134327
Informations de publication
Date de publication:
Jan 2021
Jan 2021
Historique:
received:
03
04
2020
accepted:
18
05
2020
revised:
27
04
2020
pubmed:
1
6
2020
medline:
29
6
2021
entrez:
1
6
2020
Statut:
ppublish
Résumé
Early diagnosis in primary care settings can increase access to therapies and their efficiency as well as reduce health care costs. In this context, we report in this paper the development of a disposable immunoplatform for the rapid and simultaneous determination of two protein biomarkers recently reported to be involved in the pathological process of neurodegenerative disorders (NDD), tau protein (tau), and TAR DNA-binding protein 43 (TDP-43). The methodology involves implementation of a sandwich-type immunoassay on the surface of dual screen-printed carbon electrodes (dSPCEs) electrochemically grafted with p-aminobenzoic acid (p-ABA), which allows the covalent immobilization of a gold nanoparticle-poly(amidoamine) (PAMAM) dendrimer nanocomposite (3D-Au-PAMAM). This scaffold was employed for the immobilization of the capture antibodies (CAbs). Detector antibodies labeled with horseradish peroxidase (HRP) and amperometric detection at - 0.20 V (vs. Ag pseudo-reference electrode) using the H
Identifiants
pubmed: 32474723
doi: 10.1007/s00216-020-02724-3
pii: 10.1007/s00216-020-02724-3
doi:
Substances chimiques
Biomarkers
0
DNA-Binding Proteins
0
Dendrimers
0
MAPT protein, human
0
Poly(amidoamine)
0
Polyamines
0
TARDBP protein, human
0
tau Proteins
0
Gold
7440-57-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
799-811Subventions
Organisme : Ministerio de Ciencia, Innovación y Universidades
ID : RTI2018-096135-B-I00
Organisme : Secretaría de Estado de Investigación, Desarrollo e Innovación
ID : CTQ2015-64402-C2-1-R
Organisme : Comunidad de Madrid
ID : S2018/NMT-4349
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