Functional Expression and Characterization of a Panel of Cobalt and Iron-Dependent Nitrile Hydratases.
active pharmaceutical ingredient.
amide
biocatalysis
metalloprotein
nitrile
nitrile hydratase (NHase)
non-corrinoid cobalt
non-heme iron
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
28 May 2020
28 May 2020
Historique:
received:
07
05
2020
revised:
25
05
2020
accepted:
26
05
2020
entrez:
3
6
2020
pubmed:
3
6
2020
medline:
2
3
2021
Statut:
epublish
Résumé
Nitrile hydratases (NHase) catalyze the hydration of nitriles to the corresponding amides. We report on the heterologous expression of various nitrile hydratases. Some of these enzymes have been investigated by others and us before, but sixteen target proteins represent novel sequences. Of 21 target sequences, 4 iron and 16 cobalt containing proteins were functionally expressed from
Identifiants
pubmed: 32481666
pii: molecules25112521
doi: 10.3390/molecules25112521
pmc: PMC7321127
pii:
doi:
Substances chimiques
Metalloproteins
0
Cobalt
3G0H8C9362
Iron
E1UOL152H7
Hydro-Lyases
EC 4.2.1.-
nitrile hydratase
EC 4.2.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Österreichische Forschungsförderungsgesellschaft
ID : 824186
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