Karyopherin Kap114p-mediated trans-repression controls ribosomal gene expression under saline stress.
TATA-box-binding protein
X-ray crystallography
high salinity
karyopherin-β
nucleocytoplasmic transport
Journal
EMBO reports
ISSN: 1469-3178
Titre abrégé: EMBO Rep
Pays: England
ID NLM: 100963049
Informations de publication
Date de publication:
03 07 2020
03 07 2020
Historique:
received:
18
04
2019
revised:
16
04
2020
accepted:
30
04
2020
pubmed:
3
6
2020
medline:
28
4
2021
entrez:
3
6
2020
Statut:
ppublish
Résumé
Nuclear accessibility of transcription factors controls gene expression, co-regulated by Ran-dependent nuclear localization and a competitive regulatory network. Here, we reveal that nuclear import factor-facilitated transcriptional repression attenuates ribosome biogenesis under chronic salt stress. Kap114p, one of the karyopherin-βs (Kap-βs) that mediates nuclear import of yeast TATA-binding protein (yTBP), exhibits a yTBP-binding affinity four orders of magnitude greater than its counterparts and suppresses binding of yTBP with DNA. Our crystal structure of Kap114p reveals an extensively negatively charged concave surface, accounting for high-affinity basic-protein binding. KAP114 knockout in yeast leads to a high-salt growth defect, with transcriptomic analyses revealing that Kap114p modulates expression of genes associated with ribosomal biogenesis by suppressing yTBP binding to target promoters, a trans-repression mechanism we attribute to reduced nuclear Ran levels under salinity stress. Our findings reveal that Ran integrates the nuclear transport pathway and transcription regulatory network, allowing yeast to respond to environmental stresses.
Identifiants
pubmed: 32484313
doi: 10.15252/embr.201948324
pmc: PMC7332979
doi:
Substances chimiques
Karyopherins
0
Nuclear Proteins
0
Saccharomyces cerevisiae Proteins
0
beta Karyopherins
0
Banques de données
PDB
['6AHO']
GEO
['GSE127178']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e48324Subventions
Organisme : Ministry of Science and Technology, Taiwan
ID : MOST-106-2311-B-001-038-MY3
Pays : International
Organisme : Ministry of Science and Technology, Taiwan
ID : MOST-107-2320-B-010-024-MY3
Pays : International
Organisme : Academia Sinica
ID : AS-CDA-106-L02
Pays : International
Informations de copyright
© 2020 The Authors.
Références
J Mol Biol. 1997 Aug 8;271(1):61-75
pubmed: 9300055
EMBO J. 2002 Dec 2;21(23):6527-38
pubmed: 12456659
Science. 2002 Oct 25;298(5594):799-804
pubmed: 12399584
J Biol Chem. 2006 Mar 10;281(10):6404-12
pubmed: 16407318
J Appl Crystallogr. 2017 Jun 26;50(Pt 4):1212-1225
pubmed: 28808438
EMBO Rep. 2020 Jul 3;21(7):e48324
pubmed: 32484313
Cell. 1998 Sep 4;94(5):573-83
pubmed: 9741622
FEBS Lett. 2000 Feb 25;468(2-3):149-54
pubmed: 10692576
Elife. 2017 Nov 07;6:
pubmed: 29111974
Trends Biochem Sci. 1999 Nov;24(11):437-40
pubmed: 10542411
Genes Dev. 1993 May;7(5):844-56
pubmed: 8491381
Nature. 1996 May 9;381(6578):127-51
pubmed: 8610010
Nat Struct Mol Biol. 2013 Aug;20(8):1008-14
pubmed: 23851461
Nat Struct Mol Biol. 2019 Sep;26(9):783-791
pubmed: 31439941
Nat Genet. 2001 Aug;28(4):327-34
pubmed: 11455386
J Biol Chem. 1995 Jun 9;270(23):13842-9
pubmed: 7775442
Protein Expr Purif. 2017 May;133:50-56
pubmed: 28259734
J Struct Biol. 2011 Mar;173(3):461-71
pubmed: 20920583
Elife. 2019 Mar 11;8:
pubmed: 30855230
Trends Cell Biol. 2019 Sep;29(9):752-763
pubmed: 31300188
Genes Dev. 1994 Aug 15;8(16):1920-34
pubmed: 7958867
Methods Enzymol. 1997;276:307-26
pubmed: 27754618
Nature. 2004 Dec 16;432(7019):872-7
pubmed: 15602554
J Cell Biol. 2004 Jun 7;165(5):617-23
pubmed: 15184398
Cell. 1997 Sep 19;90(6):1061-71
pubmed: 9323134
Nat Rev Immunol. 2010 May;10(5):365-76
pubmed: 20414208
Nat Rev Mol Cell Biol. 2015 Mar;16(3):129-43
pubmed: 25693126
PLoS One. 2011 Apr 27;6(4):e19308
pubmed: 21556326
Curr Opin Cell Biol. 2001 Jun;13(3):310-9
pubmed: 11343901
J Biol Chem. 1994 Mar 18;269(11):8280-6
pubmed: 8132551
J Phys Chem Lett. 2018 Aug 2;9(15):4287-4293
pubmed: 29999328
J Phys Chem Lett. 2017 Jan 19;8(2):470-477
pubmed: 28067527
Genes Dev. 1994 Oct 1;8(19):2313-23
pubmed: 7958898
Curr Opin Struct Biol. 2001 Dec;11(6):703-15
pubmed: 11751052
J Biol Chem. 2011 Aug 19;286(33):29325-35
pubmed: 21708948
Proc Natl Acad Sci U S A. 1999 Oct 26;96(22):12542-7
pubmed: 10535958
Mol Cell. 2012 Feb 24;45(4):439-46
pubmed: 22365827
Mol Cell. 2002 Oct;10(4):871-82
pubmed: 12419230
Mol Biosyst. 2015 Aug;11(8):2144-51
pubmed: 26088262
Trends Biochem Sci. 2010 Jun;35(6):309-14
pubmed: 20176488
Mol Cell Biol. 2007 Dec;27(23):8364-73
pubmed: 17908798
Mol Cell Biol. 2005 Mar;25(5):1764-78
pubmed: 15713633
J Mol Biol. 2016 May 22;428(10 Pt A):2025-39
pubmed: 26519791
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Cell. 2001 Jul 13;106(1):71-81
pubmed: 11461703
Mol Cell. 2004 May 21;14(4):479-89
pubmed: 15149597
Mol Cell Biol. 2013 Dec;33(24):4996-5004
pubmed: 24144978
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
J Mol Biol. 2004 Jun 11;339(4):681-93
pubmed: 15165843
Gene. 2003 Oct 2;315:1-13
pubmed: 14557059
J Mol Biol. 2016 May 22;428(10 Pt A):2060-90
pubmed: 26523678
Mol Biol Cell. 2000 Dec;11(12):4241-57
pubmed: 11102521
Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8174-8
pubmed: 8367480
EMBO J. 2012 May 30;31(11):2461-72
pubmed: 22562154
Eur J Cell Biol. 2004 Oct;83(10):511-20
pubmed: 15679097
EMBO J. 2003 Jun 16;22(12):3131-41
pubmed: 12805227
J Cell Biol. 1999 Jun 28;145(7):1407-17
pubmed: 10385521
Genes Dev. 2008 Sep 1;22(17):2353-8
pubmed: 18703680
Cell. 2004 Dec 29;119(7):969-79
pubmed: 15620355
Nucleic Acids Res. 2016 Jul 8;44(W1):W424-9
pubmed: 27151198
Mol Biol Cell. 2001 Feb;12(2):323-37
pubmed: 11179418
Nature. 1992 Nov 5;360(6399):40-6
pubmed: 1436073