Imaging Flow Cytometry Analysis of CEACAM Binding to Opa-Expressing Neisseria gonorrhoeae.
Neisseria gonorrhoeae
Opa protein
carcinoembryonic antigen-related cell adhesion molecule (CEACAM)
imaging flow cytometry
receptor-ligand binding
Journal
Cytometry. Part A : the journal of the International Society for Analytical Cytology
ISSN: 1552-4930
Titre abrégé: Cytometry A
Pays: United States
ID NLM: 101235694
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
20
12
2019
revised:
04
03
2020
accepted:
03
04
2020
pubmed:
3
6
2020
medline:
30
7
2021
entrez:
3
6
2020
Statut:
ppublish
Résumé
Human carcinoembryonic antigen-related cell adhesion molecules (CEACAMs) are a family of receptors that mediate intercellular interactions. Pathogenic bacteria have ligands that bind CEACAMs on human cells. Neisseria gonorrhoeae (Gc) encodes numerous unique outer membrane opacity-associated (Opa) proteins that are ligands for one or more CEACAMs. CEACAMs that are expressed on epithelial cells facilitate Gc colonization, while those expressed on neutrophils affect phagocytosis and consequent intracellular survival of Gc. Since Opa protein expression is phase-variable, variations in receptor tropism affect how individual bacteria within a population interact with host cells. Here we report the development of a rapid, quantitative method for collecting and analyzing fluorescence intensity data from thousands of cells in a population using imaging flow cytometry to detect N-CEACAM bound to the surface of Opa-expressing Gc. We use this method to confirm previous findings regarding Opa-CEACAM interactions and to examine the receptor-ligand interactions of Gc expressing other Opa proteins, as well as for other N-CEACAM proteins. © 2020 International Society for Advancement of Cytometry.
Identifiants
pubmed: 32484607
doi: 10.1002/cyto.a.24037
pmc: PMC8062897
mid: NIHMS1655245
doi:
Substances chimiques
Antigens, Bacterial
0
Bacterial Outer Membrane Proteins
0
Cell Adhesion Molecules
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1081-1089Subventions
Organisme : NIAID NIH HHS
ID : R01 AI097312
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM131829
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007046
Pays : United States
Informations de copyright
© 2020 International Society for Advancement of Cytometry.
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