Enhancing the thermostability of Rhizopus chinensis lipase by rational design and MD simulations.
Molecular dynamics simulations
Rational design
Rhizopus chinensis lipase
Thermostability
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Oct 2020
01 Oct 2020
Historique:
received:
10
03
2020
revised:
17
05
2020
accepted:
27
05
2020
pubmed:
3
6
2020
medline:
9
3
2021
entrez:
3
6
2020
Statut:
ppublish
Résumé
To improve the thermostability of r27RCL from Rhizopus chinensis and broaden its industrial applications, we used rational design (FoldX) according to ΔΔG calculation to predict mutations. Four thermostable variants S142A, D217V, Q239F, and S250Y were screened out and then combined together to generate a quadruple-mutation (S142A/D217V/Q239F/S250Y) variant, called m31. m31 exhibited enhanced thermostability with a 41.7-fold longer half-life at 60 °C, a 5 °C higher of t
Identifiants
pubmed: 32485250
pii: S0141-8130(20)33412-7
doi: 10.1016/j.ijbiomac.2020.05.243
pii:
doi:
Substances chimiques
Fungal Proteins
0
Lipase
EC 3.1.1.3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1189-1200Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare no conflicts of interest.