Advanced strategy to exploit wine-making waste by manufacturing antioxidant and prebiotic fibre-enriched vesicles for intestinal health.
Animals
Antioxidants
/ administration & dosage
Caco-2 Cells
Cell Survival
/ drug effects
Dietary Fiber
/ administration & dosage
Female
Gastrointestinal Microbiome
/ drug effects
Humans
Intestines
/ drug effects
Mice
Mice, Inbred BALB C
Oxidative Stress
/ drug effects
Particle Size
Prebiotics
/ administration & dosage
Surface Properties
Waste Products
/ analysis
Wine
/ analysis
Antioxidant activity
Grape pomace
Gut microbiota
In vivo studies
Intestinal cells
Nutriosomes
Phospholipid vesicles
Prebiotic activity
Journal
Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Titre abrégé: Colloids Surf B Biointerfaces
Pays: Netherlands
ID NLM: 9315133
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
30
04
2020
revised:
16
05
2020
accepted:
18
05
2020
pubmed:
3
6
2020
medline:
7
4
2021
entrez:
3
6
2020
Statut:
ppublish
Résumé
Grape extract-loaded fibre-enriched vesicles, nutriosomes, were prepared by combining antioxidant extracts obtained from grape pomaces and a prebiotic, soluble fibre (Nutriose®FM06). The nutriosomes were small in size (from ∼140 to 260 nm), homogeneous (polydispersity index < 0.2) and highly negative (∼ -79 mV). The vesicles were highly stable during 12 months of storage at 25 °C. When diluted with warmed (37 °C) acidic medium (pH 1.2) of high ionic strength, the vesicles only displayed an increase of the mean diameter and a low release of the extract, which were dependent on Nutriose concentration. The formulations were highly biocompatible and able to protect intestinal cells (Caco-2) from oxidative stress damage. In vivo results underlined that the composition of mouse microbiota was not affected by the vesicular formulations. Overall results support the potential application of grape nutriosomes as an alternative strategy for the protection of the intestinal tract.
Identifiants
pubmed: 32485579
pii: S0927-7765(20)30502-6
doi: 10.1016/j.colsurfb.2020.111146
pii:
doi:
Substances chimiques
Antioxidants
0
Dietary Fiber
0
Prebiotics
0
Waste Products
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
111146Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.