Plasma-derived extracellular vesicles from Plasmodium vivax patients signal spleen fibroblasts via NF-kB facilitating parasite cytoadherence.

Animals Cell Adhesion Cell-Derived Microparticles Disease Models, Animal Extracellular Vesicles / metabolism Fibroblasts / metabolism Host-Parasite Interactions / physiology Humans Intercellular Adhesion Molecule-1 / metabolism Malaria, Vivax / parasitology Male Mice Mice, Inbred C57BL Microvessels / parasitology NF-kappa B / metabolism Plasma Plasmodium vivax / physiology Proteomics Reticulocytes / metabolism Spleen / metabolism

Journal

Nature communications

ISSN: 2041-1723

Titre abrégé: Nat Commun

Pays: England

ID NLM: 101528555

Informations de publication

Date de publication:
02 06 2020

Historique:

received: 30 06 2019

accepted: 25 04 2020

entrez: 4 6 2020

pubmed: 4 6 2020

medline: 25 8 2020

Statut: epublish

Résumé

Plasmodium vivax is the most widely distributed human malaria parasite. Previous studies have shown that circulating microparticles during P. vivax acute attacks are indirectly associated with severity. Extracellular vesicles (EVs) are therefore major components of circulating plasma holding insights into pathological processes. Here, we demonstrate that plasma-derived EVs from Plasmodium vivax patients (PvEVs) are preferentially uptaken by human spleen fibroblasts (hSFs) as compared to the uptake of EVs from healthy individuals. Moreover, this uptake induces specific upregulation of ICAM-1 associated with the translocation of NF-kB to the nucleus. After this uptake, P. vivax-infected reticulocytes obtained from patients show specific adhesion properties to hSFs, reversed by inhibiting NF-kB translocation to the nucleus. Together, these data provide physiological EV-based insights into the mechanisms of human malaria pathology and support the existence of P. vivax-adherent parasite subpopulations in the microvasculature of the human spleen.

Identifiants

DOI: 10.1038/s41467-020-16337-y PMID: 32487994 PMC: PMC7265481

pubmed: 32487994

doi: 10.1038/s41467-020-16337-y

pii: 10.1038/s41467-020-16337-y

pmc: PMC7265481

doi:

Substances chimiques

ICAM1 protein, human 0

NF-kappa B 0

Intercellular Adhesion Molecule-1 126547-89-5

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

Pagination

2761

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