Evaluation of antibiotic resistance dissemination by wastewater treatment plant effluents with different catchment areas in Germany.
Acinetobacter baumannii
/ drug effects
Anti-Bacterial Agents
/ pharmacology
Bacteria
/ genetics
Drug Resistance, Microbial
/ genetics
Environmental Monitoring
/ methods
Escherichia coli
/ drug effects
Genes, Bacterial
/ drug effects
Germany
Klebsiella pneumoniae
/ drug effects
Pseudomonas aeruginosa
/ drug effects
Waste Disposal, Fluid
/ methods
Wastewater
/ microbiology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
02 06 2020
02 06 2020
Historique:
received:
18
02
2020
accepted:
07
05
2020
entrez:
4
6
2020
pubmed:
4
6
2020
medline:
6
1
2021
Statut:
epublish
Résumé
The study quantified the abundances of antibiotic resistance genes (ARGs) and facultative pathogenic bacteria (FPB) as well as one mobile genetic element in genomic DNA via qPCR from 23 different wastewater treatment plant (WWTP) effluents in Germany. 12 clinically relevant ARGs were categorized into frequently, intermediately, and rarely occurring genetic parameters of communal wastewaters. Taxonomic PCR quantifications of five FPB targeting Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Acinetobacter baumannii, and enterococci were performed. The WWTPs differed in their catchment areas being impacted by hospitals, food processing companies, or housing areas only. The total discharges of the analyzed ARGs and FPB were found to cluster independently of the sizes of the WWTPs with a maximum difference of two log units within one cluster. Initially, quantitative data evaluations revealed no significant difference between ARG categories and WWTP catchment areas. More distinct correlations became obvious with a Pearson correlation approach, where each single taxonomic marker is compared to each ARG target. Here, increased correlation of FPB (i.e. E. coli, K. pneumoniae, P. aeruginosa, and enterococci) with clinically relevant ARGs of the category of rarely occurring resistance genes (bla
Identifiants
pubmed: 32488142
doi: 10.1038/s41598-020-65635-4
pii: 10.1038/s41598-020-65635-4
pmc: PMC7265433
doi:
Substances chimiques
Anti-Bacterial Agents
0
Waste Water
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
8952Références
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