Functional characteristics of Svl3 and Pam1 that are required for proper cell wall formation in yeast cells.
2-dehydropantoate 2-reductase
C. glabrata
Pam1
S. cerevisiae
Svl3
cell wall
Journal
Yeast (Chichester, England)
ISSN: 1097-0061
Titre abrégé: Yeast
Pays: England
ID NLM: 8607637
Informations de publication
Date de publication:
07 2020
07 2020
Historique:
received:
05
02
2020
revised:
19
05
2020
accepted:
01
06
2020
pubmed:
4
6
2020
medline:
4
5
2021
entrez:
4
6
2020
Statut:
ppublish
Résumé
In the budding yeast Saccharomyces cerevisiae, Svl3 and Pam1 proteins work as functional homologues. Loss of their function causes increased levels of chitin deposition in the cell wall and temperature sensitivity, suggesting their involvement in cell wall formation. We found that the N- and C-termini of these proteins have distinctive and critical functions. They contain an N-terminal part that has a probable 2-dehydropantoate 2-reductase domain. In Svl3, this part can be replaced with the yeast 2-dehydropantoate 2-reductase, Pan5, suggesting that Svl3 and its homologues may be able to mediate 2-dehydropantoate 2-reductase function. On the other hand, Svl3 is recruited to the bud tip and bud neck via multiple localization signals in the C-terminal part. One of such signals is the lysine-rich region located in the C-terminal end. The function and localization of Svl3 are significantly disrupted by the loss of this lysine-rich region; however, its localization is not completely abolished by the mutation because another localization signal enables appropriate transport. Svl3 and Pam1 orthologues are found in cells across fungal species. The Svl3 orthologues of Candida glabrata can complement the loss of Svl3 and Pam1 in S. cerevisiae. C. glabrata cells lacking the SVL3 and PAM1 orthologue genes exhibit phenotypes similar to those observed in svl3∆pam1∆ S. cerevisiae cells. Thus, Svl3 homologues may be generally required for the assembly of the cell wall in fungal cells.
Substances chimiques
Fungal Proteins
0
Saccharomyces cerevisiae Proteins
0
Chitin
1398-61-4
Alcohol Oxidoreductases
EC 1.1.-
2-dehydropantoate 2-reductase
EC 1.1.1.169
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
359-371Informations de copyright
© 2020 John Wiley & Sons, Ltd.
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