The genome of the Cauliflower mosaic virus, a plant pararetrovirus, is highly methylated in the nucleus.
Cauliflower mosaic virus
cytosine methylation
evolution
plant pararetrovirus
plant-virus interaction
viral genomes
Journal
FEBS letters
ISSN: 1873-3468
Titre abrégé: FEBS Lett
Pays: England
ID NLM: 0155157
Informations de publication
Date de publication:
06 2020
06 2020
Historique:
received:
06
02
2020
revised:
04
05
2020
accepted:
13
05
2020
pubmed:
4
6
2020
medline:
22
4
2021
entrez:
4
6
2020
Statut:
ppublish
Résumé
Cytosine methylation is an important defense against invasive DNAs. Here, cytosine methylation profiles of a plant pararetrovirus, Cauliflower mosaic virus (CaMV), were investigated. Nuclear CaMV DNA is highly methylated throughout the genome including at transcription regulatory regions, but the virion DNA is unmethylated. In vitro CG methylation of the viral 35S promoter reduces transcription from the downstream gene. Although nuclear CaMV DNA is highly methylated, its transcripts are accumulated in the nucleus. The data suggest that a small population of unmethylated viral genomes produced through reverse transcription are constantly delivered back to the nucleus. Small RNA profiles suggest that methylation of the CaMV DNA may be due to de novo methylation through 21-, 22-, and 24-nt small RNAs with adenines at their 5' terminus.
Identifiants
pubmed: 32492176
doi: 10.1002/1873-3468.13852
doi:
Substances chimiques
RNA, Viral
0
Cytosine
8J337D1HZY
Adenine
JAC85A2161
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1974-1988Informations de copyright
© 2020 Federation of European Biochemical Societies.
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