Evidence for mutations in SARS-CoV-2 Italian isolates potentially affecting virus transmission.
Amino Acid Substitution
COVID-19
/ epidemiology
China
/ epidemiology
Coronavirus Nucleocapsid Proteins
/ chemistry
Evolution, Molecular
Genome, Viral
Humans
Italy
/ epidemiology
Models, Molecular
Molecular Epidemiology
Mutation
Pandemics
Phosphoproteins
/ chemistry
Phylogeny
Protein Conformation, alpha-Helical
Protein Conformation, beta-Strand
SARS-CoV-2
/ classification
Severity of Illness Index
Spike Glycoprotein, Coronavirus
/ chemistry
Travel
Virus Replication
COVID-19
SARS coronavirus
bioinformatics
molecular evolution
mutation
Journal
Journal of medical virology
ISSN: 1096-9071
Titre abrégé: J Med Virol
Pays: United States
ID NLM: 7705876
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
13
05
2020
accepted:
28
05
2020
pubmed:
4
6
2020
medline:
30
12
2020
entrez:
4
6
2020
Statut:
ppublish
Résumé
Italy is the first western country suffering heavy severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission and disease impact after coronavirus disease-2019 pandemia started in China. Even though the presence of mutations on spike glycoprotein and nucleocapsid in Italian isolates has been reported, the potential impact of these mutations on viral transmission has not been evaluated. We have compared SARS-CoV-2 genome sequences from Italian patients with virus sequences from Chinese patients. We focussed upon three nonsynonymous mutations of genes coding for S(one) and N (two) viral proteins present in Italian isolates and absent in Chinese ones, using various bioinformatics tools. Amino acid analysis and changes in three-dimensional protein structure suggests the mutations reduce protein stability and, particularly for S1 mutation, the enhanced torsional ability of the molecule could favor virus binding to cell receptor(s). This theoretical interpretation awaits experimental and clinical confirmation.
Identifiants
pubmed: 32492183
doi: 10.1002/jmv.26104
pmc: PMC7300971
doi:
Substances chimiques
Coronavirus Nucleocapsid Proteins
0
Phosphoproteins
0
Spike Glycoprotein, Coronavirus
0
nucleocapsid phosphoprotein, SARS-CoV-2
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2232-2237Informations de copyright
© 2020 Wiley Periodicals LLC.
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