Role of the pyruvate metabolic network on carbohydrate metabolism and virulence in Streptococcus pneumoniae.


Journal

Molecular microbiology
ISSN: 1365-2958
Titre abrégé: Mol Microbiol
Pays: England
ID NLM: 8712028

Informations de publication

Date de publication:
10 2020
Historique:
received: 06 02 2020
revised: 27 04 2020
accepted: 22 05 2020
pubmed: 5 6 2020
medline: 14 7 2021
entrez: 5 6 2020
Statut: ppublish

Résumé

Streptococcus pneumoniae is a major human pathogen that must adapt to unique nutritional environments in several host niches. The pneumococcus can metabolize a range of carbohydrates that feed into glycolysis ending in pyruvate, which is catabolized by several enzymes. We investigated how the pneumococcus utilizes these enzymes to metabolize different carbohydrates and how this impacts survival in the host. Loss of ldh decreased bacterial burden in the nasopharynx and enhanced bacteremia in mice. Loss of spxB, pdhC or pfl2 decreased bacteremia and increased host survival. In glucose or galactose, loss of ldh increased capsule production, whereas loss of spxB and pdhC reduced capsule production. The pfl2 mutant exhibited reduced capsule production only in galactose. In glucose, pyruvate was metabolized primarily by LDH to generate lactate and NAD

Identifiants

pubmed: 32495474
doi: 10.1111/mmi.14557
pmc: PMC8538403
mid: NIHMS1741751
doi:

Substances chimiques

Bacterial Proteins 0
Carbohydrates 0
Pyruvic Acid 8558G7RUTR
Hydrogen Peroxide BBX060AN9V
Galactose X2RN3Q8DNE

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

536-552

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI110618
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM034496
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI124302
Pays : United States

Informations de copyright

© 2020 John Wiley & Sons Ltd.

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Auteurs

Haley Echlin (H)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.

Matthew Frank (M)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.

Charles Rock (C)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.

Jason W Rosch (JW)

Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN, USA.

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Classifications MeSH