Polar relaxation by dynein-mediated removal of cortical myosin II.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
03 08 2020
03 08 2020
Historique:
received:
13
03
2019
revised:
03
02
2020
accepted:
04
05
2020
entrez:
5
6
2020
pubmed:
5
6
2020
medline:
23
3
2021
Statut:
ppublish
Résumé
Nearly six decades ago, Lewis Wolpert proposed the relaxation of the polar cell cortex by the radial arrays of astral microtubules as a mechanism for cleavage furrow induction. While this mechanism has remained controversial, recent work has provided evidence for polar relaxation by astral microtubules, although its molecular mechanisms remain elusive. Here, using C. elegans embryos, we show that polar relaxation is achieved through dynein-mediated removal of myosin II from the polar cortexes. Mutants that position centrosomes closer to the polar cortex accelerated furrow induction, whereas suppression of dynein activity delayed furrowing. We show that dynein-mediated removal of myosin II from the polar cortexes triggers a bidirectional cortical flow toward the cell equator, which induces the assembly of the actomyosin contractile ring. These results provide a molecular mechanism for the aster-dependent polar relaxation, which works in parallel with equatorial stimulation to promote robust cytokinesis.
Identifiants
pubmed: 32497213
pii: 151836
doi: 10.1083/jcb.201903080
pmc: PMC7401816
pii:
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Actomyosin
9013-26-7
Myosin Type II
EC 3.6.1.-
Dyneins
EC 3.6.4.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Cancer Research UK
ID : 11985
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 105627/Z/14/Z
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C19769/A11985
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT101885MA
Pays : United Kingdom
Informations de copyright
© 2020 Chapa-y-Lazo et al.
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