Invasion of vaginal epithelial cells by uropathogenic Escherichia coli.
Animals
Bacterial Adhesion
Epithelial Cells
/ microbiology
Escherichia coli Infections
/ microbiology
Female
Mice
Mice, Inbred C3H
Microscopy, Fluorescence
Phagocytosis
Urinary Bladder
/ microbiology
Urinary Tract
/ microbiology
Urinary Tract Infections
/ microbiology
Uropathogenic Escherichia coli
/ pathogenicity
Vagina
/ cytology
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
04 06 2020
04 06 2020
Historique:
received:
13
07
2019
accepted:
13
05
2020
entrez:
6
6
2020
pubmed:
6
6
2020
medline:
19
8
2020
Statut:
epublish
Résumé
Host-associated reservoirs account for the majority of recurrent and oftentimes recalcitrant infections. Previous studies established that uropathogenic E. coli - the primary cause of urinary tract infections (UTIs) - can adhere to vaginal epithelial cells preceding UTI. Here, we demonstrate that diverse urinary E. coli isolates not only adhere to, but also invade vaginal cells. Intracellular colonization of the vaginal epithelium is detected in acute and chronic murine UTI models indicating the ability of E. coli to reside in the vagina following UTI. Conversely, in a vaginal colonization model, E. coli are detected inside vaginal cells and the urinary tract, indicating that vaginal colonization can seed the bladder. More critically, bacteria are identified inside vaginal cells from clinical samples from women with a history of recurrent UTI. These findings suggest that E. coli can establish a vaginal intracellular reservoir, where it may reside safely from extracellular stressors prior to causing an ascending infection.
Identifiants
pubmed: 32499566
doi: 10.1038/s41467-020-16627-5
pii: 10.1038/s41467-020-16627-5
pmc: PMC7272400
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2803Subventions
Organisme : NIAID NIH HHS
ID : T32 AI112541
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI107052
Pays : United States
Organisme : NIDDK NIH HHS
ID : K23 DK103910
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007347
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007569
Pays : United States
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