Antibacterial activity of Xenorhabdus and Photorhabdus isolated from entomopathogenic nematodes against antibiotic-resistant bacteria.
Animals
Anti-Bacterial Agents
/ isolation & purification
Methicillin-Resistant Staphylococcus aureus
/ drug effects
Microbial Sensitivity Tests
Nematoda
/ microbiology
Oxacillin
/ pharmacology
Photorhabdus
/ classification
Phylogeny
Soil
/ parasitology
Vancomycin
/ pharmacology
Xenorhabdus
/ classification
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
14
03
2020
accepted:
19
05
2020
entrez:
6
6
2020
pubmed:
6
6
2020
medline:
22
8
2020
Statut:
epublish
Résumé
Xenorhabdus and Photorhabdus, symbiotically associated with entomopathogenic nematodes (EPNs), produce a range of antimicrobial compounds. The objective of this study is to identify Xenorhabdus and Photorhabdus and their EPNs hosts, which were isolated from soil samples from Saraburi province, and study their antibacterial activity against 15 strains of drug-resistant bacteria. Fourteen isolates (6.1%), consisting of six Xenorhabdus isolates and eight Photorhabdus isolates, were obtained from 230 soil samples. Based on the BLASTN search incorporating the phylogenetic analysis of a partial recA gene, all six isolates of Xenorhabdus were found to be identical and closely related to X. stockiae. Five isolates of Photorhabdus were found to be identical and closely related to P. luminescens subsp. akhurstii. Two isolates of Photorhabdus were found to be identical and closely related to P. luminescens subsp. hainanensis. The remaining isolate of Photorhabdus was found to be identical to P. asymbiotica subsp. australis. The bacterial extracts from P. luminescens subsp. akhurstii showed strong inhibition the growth of S. aureus strain PB36 (MSRA) by disk diffusion, minimal inhibitory concentration, and minimal bactericidal concentration assay. The combination between each extract from Xenorhabdus/Photorhabdus and oxacillin or vancomycin against S. aureus strain PB36 (MRSA) exhibited no interaction on checkerboard assay. Moreover, killing curve assay of P. luminescens subsp. akhurstii extracts against S. aureus strain PB36 exhibited a steady reduction of 105 CFU/ml to 103 CFU/ml within 30 min. This study demonstrates that Xenorhabdus and Photorhabdus, showed antibacterial activity. This finding may be useful for further research on antibiotic production.
Identifiants
pubmed: 32502188
doi: 10.1371/journal.pone.0234129
pii: PONE-D-20-07403
pmc: PMC7274414
doi:
Substances chimiques
Anti-Bacterial Agents
0
Soil
0
Vancomycin
6Q205EH1VU
Oxacillin
UH95VD7V76
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0234129Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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