The use of eugenol in combination with cefotaxime and ciprofloxacin to combat ESBL-producing quinolone-resistant pathogenic Enterobacteriaceae.
Anti-Bacterial Agents
/ pharmacology
Cefotaxime
/ pharmacology
Ciprofloxacin
/ pharmacology
Drug Resistance, Multiple, Bacterial
/ drug effects
Drug Synergism
Enterobacteriaceae
/ drug effects
Eugenol
/ pharmacology
Gene Expression Regulation, Bacterial
/ drug effects
Humans
Microbial Sensitivity Tests
Quinolones
/ pharmacology
beta-Lactamases
/ biosynthesis
ESBL
SEM
efflux pump
eugenol
qRT-PCR
quinolone
synergy
Journal
Journal of applied microbiology
ISSN: 1365-2672
Titre abrégé: J Appl Microbiol
Pays: England
ID NLM: 9706280
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
24
12
2019
revised:
05
05
2020
accepted:
19
05
2020
pubmed:
6
6
2020
medline:
30
12
2020
entrez:
6
6
2020
Statut:
ppublish
Résumé
Emergence of extended-spectrum beta-lactamase (ESBL) producing with quinolone-resistant (QR) pathogenic Enterobacteriaceae augmented the need to establish therapeutic options against them. Present study aimed towards determination of synergistic combination of eugenol (EG) with cefotaxime (CTX) and ciprofloxacin (CIP) to combat against this resistance and potentiation of antibacterial drugs by EG against these bacteria. Synergistic interaction between EG and CTX/CIP (FICI: 0·08-0·5) were observed among ESBL-QR bacteria using checkerboard assay. Approximately, 2- to 1024-fold minimum inhibitory concentration value reduction and 17- to 165 030-fold dose reduction index strongly suggested synergistic interaction between EG and antibiotics. Cell viability assay showed reduction in log Reduced doses of antibiotics, bacterial morphological alterations, efflux pump down regulation, porin over expression and beta-lactamase gene inhibition of ESBL-QR bacteria by EG alone or in combination with CTX/CIP might have reversed antibiotic resistance profile of ESBL-QR bacteria. This study provided a molecular insight into action of EG and/with CTX and CIP, which might have potentiated antibiotic's activity against ESBL-QR bacteria.
Substances chimiques
Anti-Bacterial Agents
0
Quinolones
0
Eugenol
3T8H1794QW
Ciprofloxacin
5E8K9I0O4U
beta-Lactamases
EC 3.5.2.6
Cefotaxime
N2GI8B1GK7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1566-1576Subventions
Organisme : Indian Council of Medical Research
ID : 58/67/BMS-2012
Informations de copyright
© 2020 The Society for Applied Microbiology.
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