Relationship between retinal blood flow and cytokines in central retinal vein occlusion.
Aged
Aqueous Humor
/ metabolism
Blood Flow Velocity
/ physiology
Blood Pressure
/ physiology
Cytokines
/ metabolism
Female
Humans
Intercellular Adhesion Molecule-1
/ metabolism
Laser-Doppler Flowmetry
Macular Edema
/ metabolism
Male
Middle Aged
Optic Disk
/ blood supply
Placenta Growth Factor
/ metabolism
Platelet-Derived Growth Factor
/ metabolism
Prospective Studies
Receptors, Vascular Endothelial Growth Factor
/ metabolism
Regional Blood Flow
/ physiology
Retinal Vein Occlusion
/ metabolism
Retinal Vessels
/ physiology
Visual Acuity
/ physiology
Central retinal vein occlusion
Interleukin-8
Laser speckle flowgraphy
Placental growth factor
Retinal blood flow
Soluble intercellular adhesion molecule-1
Journal
BMC ophthalmology
ISSN: 1471-2415
Titre abrégé: BMC Ophthalmol
Pays: England
ID NLM: 100967802
Informations de publication
Date de publication:
05 Jun 2020
05 Jun 2020
Historique:
received:
02
07
2019
accepted:
27
05
2020
entrez:
7
6
2020
pubmed:
7
6
2020
medline:
10
4
2021
Statut:
epublish
Résumé
We evaluated the relationship between retinal blood flow and aqueous humor levels of cytokines/growth factors in patients with central retinal vein occlusion (CRVO). In an observational study, 64 eyes of 64 CRVO patients were examined before anti-vascular endothelial growth factor (VEGF) therapy. Blood flow was assessed in large vessels around and at the optic disk by determining the mean blur rate using laser speckle flowgraphy. Aqueous humor samples were obtained from the patients during anti-VEGF therapy and levels of the following molecules were measured by the suspension array method: soluble VEGF receptor (sVEGFR)-1, sVEGFR-2, VEGF, plancental growth factor (PlGF), platelet-derived growth factor (PDGF)-AA, soluble intercellular adhesion molecule (sICAM)-1, monocyte chemotactic protein (MCP)-1, interleukin (IL)-6, IL-8, IL-12(p70), and IL-13. The mean blur rate of the affected eye was significantly lower than that of the unaffected eye. The mean blur rate showed a significant negative correlation with the log-transformed aqueous humor levels of PlGF, sICAM-1, and IL-8, but not VEGF. These findings suggest that retinal blood flow velocity might be more strongly correlated with inflammatory factors than VEGF in patients with nonischemic CRVO and macular edema.
Sections du résumé
BACKGROUND
BACKGROUND
We evaluated the relationship between retinal blood flow and aqueous humor levels of cytokines/growth factors in patients with central retinal vein occlusion (CRVO).
METHODS
METHODS
In an observational study, 64 eyes of 64 CRVO patients were examined before anti-vascular endothelial growth factor (VEGF) therapy. Blood flow was assessed in large vessels around and at the optic disk by determining the mean blur rate using laser speckle flowgraphy. Aqueous humor samples were obtained from the patients during anti-VEGF therapy and levels of the following molecules were measured by the suspension array method: soluble VEGF receptor (sVEGFR)-1, sVEGFR-2, VEGF, plancental growth factor (PlGF), platelet-derived growth factor (PDGF)-AA, soluble intercellular adhesion molecule (sICAM)-1, monocyte chemotactic protein (MCP)-1, interleukin (IL)-6, IL-8, IL-12(p70), and IL-13.
RESULTS
RESULTS
The mean blur rate of the affected eye was significantly lower than that of the unaffected eye. The mean blur rate showed a significant negative correlation with the log-transformed aqueous humor levels of PlGF, sICAM-1, and IL-8, but not VEGF.
CONCLUSIONS
CONCLUSIONS
These findings suggest that retinal blood flow velocity might be more strongly correlated with inflammatory factors than VEGF in patients with nonischemic CRVO and macular edema.
Identifiants
pubmed: 32503534
doi: 10.1186/s12886-020-01486-x
pii: 10.1186/s12886-020-01486-x
pmc: PMC7273673
doi:
Substances chimiques
Cytokines
0
ICAM1 protein, human
0
PGF protein, human
0
Platelet-Derived Growth Factor
0
platelet-derived growth factor A
0
Intercellular Adhesion Molecule-1
126547-89-5
Placenta Growth Factor
144589-93-5
Receptors, Vascular Endothelial Growth Factor
EC 2.7.10.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
215Références
Open Ophthalmol J. 2012;6:63-4
pubmed: 22833773
Ophthalmology. 1997 Apr;104(4):659-63
pubmed: 9111260
Ophthalmology. 1983 May;90(5):488-506
pubmed: 6192376
J Biol Chem. 1994 Oct 14;269(41):25646-54
pubmed: 7929268
Ophthalmology. 2011 Oct;118(10):2041-9
pubmed: 21715011
BMC Ophthalmol. 2013 Dec 11;13:78
pubmed: 24325604
Am J Ophthalmol. 1983 Feb;95(2):182-8
pubmed: 6824050
Invest Ophthalmol Vis Sci. 1998 Apr;39(5):793-800
pubmed: 9538887
Proc Natl Acad Sci U S A. 1998 Sep 29;95(20):11709-14
pubmed: 9751730
Ophthalmologica. 1976;172(1):1-13
pubmed: 1250571
Retina. 2015 Oct;35(10):2037-42
pubmed: 25932555
Biochem Biophys Res Commun. 1990 Jan 15;166(1):187-92
pubmed: 2405845
Invest Ophthalmol Vis Sci. 2014 Mar 19;55(3):1625-9
pubmed: 24550362
J Exp Med. 1990 Apr 1;171(4):1155-62
pubmed: 1969919
J Clin Invest. 1996 Apr 15;97(8):1931-41
pubmed: 8621778
Nat Med. 2000 Apr;6(4):389-95
pubmed: 10742145
Ophthalmology. 1994 Aug;101(8):1362-72
pubmed: 7520147
Acta Ophthalmol. 2010 Nov;88(7):723-9
pubmed: 19725814
Proc Natl Acad Sci U S A. 1999 Sep 14;96(19):10836-41
pubmed: 10485912
Arch Ophthalmol. 1993 Aug;111(8):1087-95
pubmed: 7688950
Am J Ophthalmol. 2013 Mar;155(3):429-437.e7
pubmed: 23218699
Retina. 2018 Feb;38(2):283-291
pubmed: 28151838
Trends Cardiovasc Med. 2002 Aug;12(6):241-6
pubmed: 12242046
Am J Ophthalmol. 2000 Jun;129(6):734-9
pubmed: 10926981
Am J Ophthalmol. 2006 Jan;141(1):31-34
pubmed: 16386973
Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9267-71
pubmed: 1924389
J Biol Chem. 1996 Jul 26;271(30):17629-34
pubmed: 8663424
Lab Invest. 1992 Feb;66(2):200-11
pubmed: 1346541
Ophthalmology. 2001 Mar;108(3):627-32
pubmed: 11237920
Invest Ophthalmol Vis Sci. 2015 Jan 29;56(2):1122-8
pubmed: 25634982
Br J Ophthalmol. 2009 May;93(5):630-3
pubmed: 19208676
Arch Ophthalmol. 2003 Feb;121(2):225-31
pubmed: 12583789
Ophthalmology. 2009 Jan;116(1):87-93
pubmed: 19118700
Arch Ophthalmol. 1991 Apr;109(4):527-31
pubmed: 2012555
Invest Ophthalmol Vis Sci. 2012 Dec 17;53(13):8303-9
pubmed: 23169886
J Clin Invest. 1994 Apr;93(4):1564-70
pubmed: 8163658
Retina. 2010 Nov-Dec;30(10):1692-7
pubmed: 20706174
Mol Cell Biol. 1996 Aug;16(8):4231-9
pubmed: 8754823