A novel method for evaluating activity of transient receptor potential channels using a cellular dielectric spectroscopy.

Dielectric Spectroscopy / methods HEK293 Cells Humans TRPA1 Cation Channel TRPV Cation Channels Transient Receptor Potential Channels / metabolism
CellKey™ system Cellular dielectric spectroscopy TRPV1

Journal

Journal of pharmacological sciences
ISSN: 1347-8648
Titre abrégé: J Pharmacol Sci
Pays: Japan
ID NLM: 101167001

Informations de publication

Date de publication:
Aug 2020
Historique:
received: 09 10 2019
revised: 27 01 2020
accepted: 29 01 2020
pubmed: 9 6 2020
medline: 11 8 2020
entrez: 8 6 2020
Statut: ppublish

Résumé

Cellular dielectric spectroscopy (CDS) is a novel technology enabling pharmacological evaluation of multiple receptor types with a label-free cell-based assay. We evaluated activities of a family of ligand-gated channels, transient receptor potential vanilloid 1 (TRPV1) and transient receptor potential ankyrin 1 (TRPA1) channels by an electrical impedance-based biosensor (CellKey™ system) using CDS. Measures of both potency (EC

Identifiants

DOI: 10.1016/j.jphs.2020.05.001 PMID: 32505645
pubmed: 32505645
pii: S1347-8613(20)30045-1
doi: 10.1016/j.jphs.2020.05.001
pii:
doi:

Substances chimiques

TRPA1 Cation Channel 0
TRPV Cation Channels 0
TRPV1 receptor 0
Transient Receptor Potential Channels 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

320-324

Informations de copyright

Copyright © 2020 The Authors. Production and hosting by Elsevier B.V. All rights reserved.

Auteurs

Kanako Miyano (K)

Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan.

Katsuya Ohbuchi (K)

Tsumura Research Labs, Tumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, Japan.

Yuka Sudo (Y)

Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan.

Kouichiro Minami (K)

Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Emergency Life-Saving Technique Academy of Tokyo (ELSTA TOKYO), Hachioji-shi, Tokyo 192-0364, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, Tochigi 329-0498, Japan.

Toru Yokoyama (T)

Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Emergency Life-Saving Technique Academy of Tokyo (ELSTA TOKYO), Hachioji-shi, Tokyo 192-0364, Japan; Department of Anesthesiology and Critical Care Medicine, Jichi Medical University, Tochigi 329-0498, Japan.

Masahiro Yamamoto (M)

Tsumura Research Labs, Tumura & Co., 3586 Yoshiwara, Ami-machi, Inashiki-gun, Ibaraki 300-1192, Japan.

Miaki Uzu (M)

Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan.

Miki Nonaka (M)

Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan.

Seiji Shiraishi (S)

Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan.

Hiroaki Murata (H)

Department of Anesthesiology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8501, Japan.

Yoshikazu Higami (Y)

Laboratory of Molecular Pathology and Metabolic Disease, Faculty of Pharmaceutical Sciences, Tokyo University of Science, Chiba 278-8510, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan.

Yasuhito Uezono (Y)

Division of Cancer Pathophysiology, National Cancer Center Research Institute, Tokyo 104-0045, Japan; Translational Research Center, Research Institute of Science and Technology, Tokyo University of Science, Chiba 278-8510, Japan; Division of Supportive Care Research, Exploratory Oncology Research & Clinical Trial Center, National Cancer Center, Tokyo 104-0045, Japan; Innovation Center for Supportive, Palliative and Psychosocial Care, National Cancer Center Hospital, Tokyo 104-0045, Japan; Department of Comprehensive Oncology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki 852-8523, Japan. Electronic address: yuezono@ncc.go.jp.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectroscopie diélectrique › A novel method for evaluating activity of...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › A novel method for evaluating activity of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › A novel method for evaluating activity of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Canaux ioniques › Canaux cationiques TRP › Membre-1 de la sous-famille A de canaux cationiques à potentiel de récepteur transitoire › A novel method for evaluating activity of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de transport › Canaux cationiques TRPV › A novel method for evaluating activity of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Canaux ioniques › Canaux cationiques TRP › A novel method for evaluating activity of...