Zonisamide promotes survival of human-induced pluripotent stem cell-derived dopaminergic neurons in the striatum of female rats.
Parkinson's disease
RRID:AB_2169021
RRID:AB_2190153
RRID:AB_2201528
RRID:AB_2244867
RRID:AB_2294104
RRID:AB_2340866
RRID:AB_2534074
RRID:AB_2534102
RRID:AB_2534105
RRID:AB_2535795
RRID:AB_2535864
RRID:AB_2556542
RRID:AB_2556546
RRID:AB_2556547
RRID:AB_2716768
RRID:AB_2801320
RRID:AB_390204
RRID:AB_442102
RRID:AB_477010
RRID:AB_90755
RRID:AB_94090
RRID:CVCL_RL23
RRID:RGD_1547866
RRID:RGD_9685748
RRID:SCR_001456
RRID:SCR_002798
RRID:SCR_010972
RRID:SCR_016264
RRID:SCR_017202
RRID:SCR_017205
RRID:SCR_017206
Zonisamide
dopaminergic neuron
induced pluripotent stem cell
transplantation
Journal
Journal of neuroscience research
ISSN: 1097-4547
Titre abrégé: J Neurosci Res
Pays: United States
ID NLM: 7600111
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
23
08
2019
revised:
15
05
2020
accepted:
17
05
2020
pubmed:
9
6
2020
medline:
24
8
2021
entrez:
8
6
2020
Statut:
ppublish
Résumé
The transplantation of dopaminergic (DA) progenitors derived from pluripotent stem cells improves the behavior of Parkinson's disease model animals. However, the survival of DA progenitors is low, and the final yield of DA neurons is only approximately 0.3%-2% the number of transplanted cells. Zonisamide (ZNS) increases the number of survived DA neurons upon the transplantation of mouse-induced pluripotent stem (iPS) cell-derived DA progenitors in the rat striatum. In this study, we induced DA progenitors from human iPS cells and transplanted them into the striatum of female rats with daily administration of ZNS. The number of survived DA neurons was evaluated 1 and 4 months after transplantation by immunohistochemistry, which revealed that the number of survived DA neurons was significantly increased with the administration of ZNS. To assess the mechanism of action of ZNS, we performed a gene expression analysis to compare the gene expression profiles in striatum treated with or without ZNS. The analysis revealed that the expression of SLIT-and NTRK-like protein 6 (SLITRK6) was upregulated in rat striatum treated with ZNS. In conclusion, ZNS promotes the survival of DA neurons after the transplantation of human-iPS cell-derived DA progenitors in the rat striatum. SLITRK6 is suggested to be involved in this supportive effect of ZNS by modulating the environment of the host brain.
Identifiants
pubmed: 32506530
doi: 10.1002/jnr.24668
pmc: PMC7497107
doi:
Substances chimiques
Membrane Proteins
0
Slitrk6 protein, human
0
Zonisamide
459384H98V
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1575-1587Informations de copyright
© 2020 Wiley Periodicals, Inc.
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