Cytochrome P450-mediated N-demethylation of noscapine by whole-cell biotransformation: process limitations and strategies for optimisation.
Bacillus megaterium
/ metabolism
Biotransformation
Catalysis
Computer Simulation
Cyclodextrins
/ chemistry
Cytochrome P-450 Enzyme System
/ metabolism
Demethylation
Escherichia coli
/ metabolism
Industrial Microbiology
/ methods
Mutation
Noscapine
/ chemistry
Organic Chemicals
/ metabolism
Oxidation-Reduction
Polymers
/ chemistry
Solubility
Solvents
Bacillus megaterium
Biphasic
Cyclodextrin
Mass transfer
P450BM3
Journal
Journal of industrial microbiology & biotechnology
ISSN: 1476-5535
Titre abrégé: J Ind Microbiol Biotechnol
Pays: Germany
ID NLM: 9705544
Informations de publication
Date de publication:
Jul 2020
Jul 2020
Historique:
received:
17
02
2020
accepted:
29
05
2020
pubmed:
9
6
2020
medline:
17
12
2020
entrez:
9
6
2020
Statut:
ppublish
Résumé
Cytochrome P450 enzymes catalyse reactions of significant industrial interest but are underutilised in large-scale bioprocesses due to enzyme stability, cofactor requirements and the poor aqueous solubility and microbial toxicity of typical substrates and products. In this work, we investigate the potential for preparative-scale N-demethylation of the opium poppy alkaloid noscapine by a P450
Identifiants
pubmed: 32507955
doi: 10.1007/s10295-020-02283-7
pii: 10.1007/s10295-020-02283-7
doi:
Substances chimiques
Cyclodextrins
0
Organic Chemicals
0
Polymers
0
Solvents
0
Noscapine
8V32U4AOQU
Cytochrome P-450 Enzyme System
9035-51-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM