Description of an atypical Toxoplasma gondii isolate from a case of congenital toxoplasmosis in northeastern Brazil.
Genotyping
Mouse bioassay
Newborn
Virulence
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
22
11
2019
accepted:
01
06
2020
pubmed:
11
6
2020
medline:
23
9
2020
entrez:
11
6
2020
Statut:
ppublish
Résumé
We report a case of severe congenital toxoplasmosis that involved an atypical T. gondii genotype in a newborn baby from Alagoas state in Northeastern Brazil. A pregnant woman presented IgM and IgG anti-T. gondii antibodies, as detected by the chemiluminescence immunoassay on the second trimester of pregnancy. A mouse bioassay was performed using umbilical cord blood and one isolate was obtained. The isolate was designated TgCTBrAL1 and genetic characterization revealed genotype ToxoDB #162. Genotype results of the rhoptry genes, ROP5 and ROP18, could predict the high virulence of the isolate in mice, which was confirmed by an in vivo virulence assay. This is the first report of generating a T. gondii isolate from a newborn baby with congenital toxoplasmosis in Northeastern Brazil.
Identifiants
pubmed: 32518965
doi: 10.1007/s00436-020-06746-9
pii: 10.1007/s00436-020-06746-9
doi:
Substances chimiques
Protozoan Proteins
0
Types de publication
Case Reports
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2727-2731Subventions
Organisme : Conselho Nacional de Desenvolvimento Científico e Tecnológico
ID : 206740/2017-4
Organisme : Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco
ID : IBPG-1358-5.05./15
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