A genetic polymorphism of IL17F rs763780 associated with anti-E production in the Han Chinese population.
E-alloimmunization
IL17F
Single nucleotide polymorphisms
Journal
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis
ISSN: 1473-0502
Titre abrégé: Transfus Apher Sci
Pays: England
ID NLM: 101095653
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
19
12
2019
revised:
04
02
2020
accepted:
19
02
2020
pubmed:
12
6
2020
medline:
9
6
2021
entrez:
12
6
2020
Statut:
ppublish
Résumé
This study aimed to investigate the association among 4 single nucleotide polymorphisms (SNPs) in the genes TLR3, IL17F, ERAP1 and ERAP2 with anti-E alloantibody production. Anti-E alloantibodies can lead to clinically significant delayed hemolytic transfusion reactions (DHTRs) and hemolytic disease of the newborn (HDN). Some individuals produce anti-E alloantibodies post- transfusion. The mechanisms controlling this process is poorly understood. Ninety-five patients with anti-E alloantibodies were enrolled, and samples from 186 healthy donors were used as controls. Four SNPs in the immune-related genes (TLR3, IL17F, ERAP1 and ERAP2) were selected. SNPs were analyzed by polymerase chain reactions (PCR) and TaqMan assays. Allele and genotype frequencies were compared using Pearson's chi-square test. The C allele and CC + CT genotypes of rs763780 in the IL17F gene were overrepresented in the E- alloimmunized patient group (14.2 % vs. 5.1 %, P < 0.001; 23.2 % vs. 9.7 %; P = 0.004). Individuals with CC + CT genotypes of rs763780 had a higher risk of E-alloimmunization. (OR, 2.81; 95 % CI, 1.42-5.56). No significant difference was observed among the other 3 SNPs. SNP rs763780 in the IL17F gene was associated with E-alloimmunization in a sample of the Han Chinese population, with the allele C as a risk allele.
Sections du résumé
OBJECTIVE
OBJECTIVE
This study aimed to investigate the association among 4 single nucleotide polymorphisms (SNPs) in the genes TLR3, IL17F, ERAP1 and ERAP2 with anti-E alloantibody production.
BACKGROUND
BACKGROUND
Anti-E alloantibodies can lead to clinically significant delayed hemolytic transfusion reactions (DHTRs) and hemolytic disease of the newborn (HDN). Some individuals produce anti-E alloantibodies post- transfusion. The mechanisms controlling this process is poorly understood.
METHODS
METHODS
Ninety-five patients with anti-E alloantibodies were enrolled, and samples from 186 healthy donors were used as controls. Four SNPs in the immune-related genes (TLR3, IL17F, ERAP1 and ERAP2) were selected. SNPs were analyzed by polymerase chain reactions (PCR) and TaqMan assays. Allele and genotype frequencies were compared using Pearson's chi-square test.
RESULTS
RESULTS
The C allele and CC + CT genotypes of rs763780 in the IL17F gene were overrepresented in the E- alloimmunized patient group (14.2 % vs. 5.1 %, P < 0.001; 23.2 % vs. 9.7 %; P = 0.004). Individuals with CC + CT genotypes of rs763780 had a higher risk of E-alloimmunization. (OR, 2.81; 95 % CI, 1.42-5.56). No significant difference was observed among the other 3 SNPs.
CONCLUSIONS
CONCLUSIONS
SNP rs763780 in the IL17F gene was associated with E-alloimmunization in a sample of the Han Chinese population, with the allele C as a risk allele.
Identifiants
pubmed: 32522475
pii: S1473-0502(20)30032-X
doi: 10.1016/j.transci.2020.102745
pii:
doi:
Substances chimiques
IL17F protein, human
0
Interleukin-17
0
Types de publication
Journal Article
Langues
eng
Pagination
102745Informations de copyright
Copyright © 2020. Published by Elsevier Ltd.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors have no competing interests.