Expression Patterns of Hypoxia-Inducible Factors, Proinflammatory, and Neuroprotective Cytokines in Neuroepithelial Tissues of Lumbar Spinal Lipomas-A Pilot Study.
Basic Helix-Loop-Helix Transcription Factors
/ biosynthesis
Child
Child, Preschool
Cytokines
/ biosynthesis
Female
Gene Expression Regulation, Neoplastic
Humans
Hypoxia-Inducible Factor 1, alpha Subunit
/ biosynthesis
Infant
Inflammation Mediators
/ metabolism
Lipoma
/ diagnostic imaging
Lumbar Vertebrae
/ diagnostic imaging
Male
Neoplasms, Neuroepithelial
/ diagnostic imaging
Pilot Projects
Spinal Cord Neoplasms
/ diagnostic imaging
Cytokines
Hypoxia
Immunohistochemistry
MRI
Real-time RT-PCR
Spinal lipoma
Journal
World neurosurgery
ISSN: 1878-8769
Titre abrégé: World Neurosurg
Pays: United States
ID NLM: 101528275
Informations de publication
Date de publication:
09 2020
09 2020
Historique:
received:
29
03
2020
revised:
27
05
2020
accepted:
28
05
2020
pubmed:
12
6
2020
medline:
7
1
2021
entrez:
12
6
2020
Statut:
ppublish
Résumé
Lumbosacral lipomas (LSLs), one form of closed spinal dysraphism, are congenital disorders of the terminal spinal cord (SC). Delayed neurologic deterioration often occurs in the subsequent developmental course of the patient. Identifying the cellular and molecular factors underlying the progressive damage to neural structures is a prerequisite for developing treatment strategies for LSLs. Nine LSL specimens obtained from the SC/lipoma interface during surgical resection were examined. Normal SC tissue served as a control. Clinical characteristics were obtained, and spinal magnetic resonance imaging was re-evaluated. Cellular marker profiles were established. Immunoreactivity (IR) of hypoxia-inducible factor 1α (HIF-1α/-2α), erythropoietin (Epo)/erythropoietin receptor (EpoR), interleukin-1β (IL-1β)/IL-1R1, and tumor necrosis factor α/tumor necrosis factor receptor type 1 were analyzed qualitatively and semiquantitatively by densitometry. Colabeling with cellular markers was determined by multifluorescence labeling. Cytokines were further analyzed by real-time reverse transcription polymerase chain reaction. LSL specimens showed significant gliosis. HIF-1α/HIF-2α-IR and Epo/Epo-IR were found at significantly higher levels in the LSL specimens, as were IL-1β-/IL-1β receptor type 1 (IL1-R1) and tumor necrosis factor α/tumor necrosis factor receptor type 1 (P < 0.001), than were the controls. At the messenger RNA level, cytokines appeared partially induced. Double immunofluorescence labeling confirmed the costaining of these factors with inflammatory and glial markers. The expression of hypoxia-related and inflammatory mediators was shown for the first time in LSL specimens. These factors might play a role in multifactorial secondary lesion cascades underlying further damage to the neural placode in closed dysraphism.
Identifiants
pubmed: 32522652
pii: S1878-8750(20)31229-8
doi: 10.1016/j.wneu.2020.05.256
pii:
doi:
Substances chimiques
Basic Helix-Loop-Helix Transcription Factors
0
Cytokines
0
HIF1A protein, human
0
Hypoxia-Inducible Factor 1, alpha Subunit
0
Inflammation Mediators
0
endothelial PAS domain-containing protein 1
1B37H0967P
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e633-e644Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.