Spt6 is a maintenance factor for centromeric CENP-A.
Animals
Blotting, Western
Cell Cycle
/ genetics
Cell Line
Centromere Protein A
/ genetics
Clustered Regularly Interspaced Short Palindromic Repeats
/ genetics
Drosophila
Drosophila Proteins
/ genetics
Flow Cytometry
Fluorescent Antibody Technique
HeLa Cells
Humans
Immunoprecipitation
Mitosis
/ genetics
Peptide Elongation Factors
/ genetics
Transcription Factors
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
10 06 2020
10 06 2020
Historique:
received:
04
12
2018
accepted:
19
05
2020
entrez:
12
6
2020
pubmed:
12
6
2020
medline:
25
8
2020
Statut:
epublish
Résumé
Replication and transcription of genomic DNA requires partial disassembly of nucleosomes to allow progression of polymerases. This presents both an opportunity to remodel the underlying chromatin and a danger of losing epigenetic information. Centromeric transcription is required for stable incorporation of the centromere-specific histone dCENP-A in M/G1 phase, which depends on the eviction of previously deposited H3/H3.3-placeholder nucleosomes. Here we demonstrate that the histone chaperone and transcription elongation factor Spt6 spatially and temporarily coincides with centromeric transcription and prevents the loss of old CENP-A nucleosomes in both Drosophila and human cells. Spt6 binds directly to dCENP-A and dCENP-A mutants carrying phosphomimetic residues alleviate this association. Retention of phosphomimetic dCENP-A mutants is reduced relative to wildtype, while non-phosphorylatable dCENP-A retention is increased and accumulates at the centromere. We conclude that Spt6 acts as a conserved CENP-A maintenance factor that ensures long-term stability of epigenetic centromere identity during transcription-mediated chromatin remodeling.
Identifiants
pubmed: 32522980
doi: 10.1038/s41467-020-16695-7
pii: 10.1038/s41467-020-16695-7
pmc: PMC7287101
doi:
Substances chimiques
Centromere Protein A
0
Cid protein, Drosophila
0
Drosophila Proteins
0
Peptide Elongation Factors
0
SUPT6H protein, human
0
Spt6 protein, Drosophila
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2919Subventions
Organisme : Wellcome Trust
ID : 203149
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 103897/Z/14/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 210645/Z/18/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 101527/Z/13/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 092076
Pays : United Kingdom
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