Reference Genes for Expression Studies in Human CD8
Adolescent
Adult
CD4-Positive T-Lymphocytes
/ immunology
CD8-Positive T-Lymphocytes
/ immunology
Female
Gene Expression Profiling
/ methods
Humans
Immunologic Memory
/ immunology
Interferon-gamma
/ immunology
Male
RNA, Messenger
/ immunology
Real-Time Polymerase Chain Reaction
/ methods
Reference Standards
Rest
/ physiology
Young Adult
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
10 06 2020
10 06 2020
Historique:
received:
11
04
2019
accepted:
15
05
2020
entrez:
12
6
2020
pubmed:
12
6
2020
medline:
24
11
2020
Statut:
epublish
Résumé
Reverse-transcription quantitative real-time polymerase chain reaction (RT-qPCR) is widely used for mRNA quantification. To accurately measure changing gene transcript levels under different experimental conditions, the use of appropriate reference gene transcripts is instrumental. In T cell immunology, suitable reference genes have been reported for bulk CD4
Identifiants
pubmed: 32523060
doi: 10.1038/s41598-020-66367-1
pii: 10.1038/s41598-020-66367-1
pmc: PMC7286888
doi:
Substances chimiques
RNA, Messenger
0
Interferon-gamma
82115-62-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
9411Références
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