Colistin Resistance and Extensive Genetic Variations in PmrAB and PhoPQ in Klebsiella Pneumoniae Isolates from South Korea.
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
27
11
2019
accepted:
03
06
2020
pubmed:
12
6
2020
medline:
15
5
2021
entrez:
12
6
2020
Statut:
ppublish
Résumé
We investigated the colistin resistance of Klebsiella pneumoniae blood isolates from South Korea. Among 252 K. pneumoniae isolates, only 11 (4.4%) demonstrated colistin resistance, of which, one was resistant to all antibiotics but tigecycline. Multilocus sequence typing analysis revealed ten sequence types among the 11 colistin-resistant isolates, indicating independent occurrence of colistin resistance in K. pneumoniae. To understand the mechanism of colistin resistance, amino acid variations in PmrAB, PmrD, PhoPQ, and MgrB were investigated. Amino acid substitutions were identified in all the colistin-resistant K. pneumoniae isolates. Particularly, extensive alterations in the genes associated with colistin resistance were shared in four colistin-resistant isolates, suggesting recombination between these genes of unrelated isolates. Our results suggest that genetic recombination is responsible for colistin resistance in some K. pneumoniae isolates.
Identifiants
pubmed: 32524274
doi: 10.1007/s00284-020-02074-4
pii: 10.1007/s00284-020-02074-4
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
Colistin
Z67X93HJG1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2307-2311Subventions
Organisme : National Research Foundation of Korea
ID : NRF-2019R1A2C2004879