Protective effect of L-carnitine on platelet apoptosis during storage of platelet concentrate.
Adult
Antioxidants
/ pharmacology
Apoptosis
/ drug effects
Blood Platelets
/ cytology
Blood Preservation
Carnitine
/ pharmacology
Caspase 3
/ blood
Cytochromes c
/ blood
Female
Humans
Male
Membrane Lipids
/ blood
Membrane Potential, Mitochondrial
/ drug effects
Middle Aged
Organ Preservation Solutions
/ pharmacology
Phosphatidylserines
/ blood
Platelet-Rich Plasma
Apoptosis
Caspase-3
Cytochrome C
L-carnitine
Platelet concentrate
apoptose
caspase-3
concentré de plaquettes
cytochrome C
Journal
Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine
ISSN: 1953-8022
Titre abrégé: Transfus Clin Biol
Pays: France
ID NLM: 9423846
Informations de publication
Date de publication:
Aug 2020
Aug 2020
Historique:
received:
18
02
2020
revised:
16
05
2020
accepted:
08
06
2020
pubmed:
17
6
2020
medline:
30
7
2021
entrez:
17
6
2020
Statut:
ppublish
Résumé
Platelet apoptosis is considered as one of the important factors involved in platelet storage lesion (PSL) and affect the quality of platelets during storage. The beneficial effect of L-carnitine (LC) on platelet apoptosis during platelet concentrates (PCs) storage has not been fully investigated. The aim of this study was to evaluate the effects of LC on platelets of PC regarding their apoptosis markers during storage. Ten PCs from healthy donors were investigated in this study. PCs were prepared by platelet rich plasma (PRP) method and stored at 22±2°C with gentle agitation during storage. The effects of LC (15mM) on the platelet apoptosis were assessed by analyzing different indicative presence or absence of LC. Sampling was performed to evaluate apoptosis markers during platelet storage. The results indicated significantly higher mitochondrial membrane potential for LC-treated platelets than the untreated on the days 2 and 5 of storage (P The results of this study indicate that the use of LC as an additive solution in platelets may be useful to reduce PSL by decreasing platelet apoptosis via mitochondrial pathway and increase platelet quality during storage.
Sections du résumé
BACKGROUND
BACKGROUND
Platelet apoptosis is considered as one of the important factors involved in platelet storage lesion (PSL) and affect the quality of platelets during storage. The beneficial effect of L-carnitine (LC) on platelet apoptosis during platelet concentrates (PCs) storage has not been fully investigated. The aim of this study was to evaluate the effects of LC on platelets of PC regarding their apoptosis markers during storage.
METHODS
METHODS
Ten PCs from healthy donors were investigated in this study. PCs were prepared by platelet rich plasma (PRP) method and stored at 22±2°C with gentle agitation during storage. The effects of LC (15mM) on the platelet apoptosis were assessed by analyzing different indicative presence or absence of LC. Sampling was performed to evaluate apoptosis markers during platelet storage.
RESULTS
RESULTS
The results indicated significantly higher mitochondrial membrane potential for LC-treated platelets than the untreated on the days 2 and 5 of storage (P
CONCLUSION
CONCLUSIONS
The results of this study indicate that the use of LC as an additive solution in platelets may be useful to reduce PSL by decreasing platelet apoptosis via mitochondrial pathway and increase platelet quality during storage.
Identifiants
pubmed: 32544525
pii: S1246-7820(20)30073-2
doi: 10.1016/j.tracli.2020.06.002
pii:
doi:
Substances chimiques
Antioxidants
0
Membrane Lipids
0
Organ Preservation Solutions
0
Phosphatidylserines
0
Cytochromes c
9007-43-6
CASP3 protein, human
EC 3.4.22.-
Caspase 3
EC 3.4.22.-
Carnitine
S7UI8SM58A
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
139-146Informations de copyright
Copyright © 2020 Société française de transfusion sanguine (SFTS). Published by Elsevier Masson SAS. All rights reserved.