Structural characterization and immunomodulatory activity of a polysaccharide from Eurotium cristatum.
Eurotium cristatum
Immunomodulatory
Polysaccharides
Purification
Structural characterization
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Nov 2020
01 Nov 2020
Historique:
received:
24
01
2020
revised:
30
05
2020
accepted:
11
06
2020
pubmed:
17
6
2020
medline:
25
3
2021
entrez:
17
6
2020
Statut:
ppublish
Résumé
By combining DEAE-cellulose-52 and Sepharose-CL-6B chromatography, a heteropolysaccharide ECIP-1A with an average molecular weight of 24,336 Da was isolated from the hyphae of Eurotium cristatum. The main components of the monosaccharides of ECIP-1A were mannose, glucose and galactose, and their molar ratio is 4.003: 0.592: 4.58. Analysis of the immunomodulatory activity showed that ECIP-1A could significantly promote the proliferation of RAW264.7 cells and induce the production of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and nitric oxide (NO) by RAW264.7 cells. The results therefore suggested that ECIP-1A, as an immunomodulator, may have potential application prospects in the food and pharmaceutical industries.
Identifiants
pubmed: 32544589
pii: S0141-8130(20)33540-6
doi: 10.1016/j.ijbiomac.2020.06.099
pii:
doi:
Substances chimiques
Fungal Polysaccharides
0
Immunologic Factors
0
Interleukin-6
0
Tumor Necrosis Factor-alpha
0
interleukin-6, mouse
0
Nitric Oxide
31C4KY9ESH
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
609-617Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no conflict of interest.