HIP1R acts as a tumor suppressor in gastric cancer by promoting cancer cell apoptosis and inhibiting migration and invasion through modulating Akt.
Adaptor Proteins, Signal Transducing
/ genetics
Apoptosis
Biomarkers, Tumor
/ genetics
Cell Movement
Cell Proliferation
Female
Gene Expression Regulation, Neoplastic
Humans
Male
Microfilament Proteins
/ genetics
Middle Aged
Neoplasm Invasiveness
Prognosis
Proto-Oncogene Proteins c-akt
/ genetics
Stomach Neoplasms
/ genetics
Survival Rate
Tumor Cells, Cultured
apoptosis
gastric cancer
huntingtin-interacting protein 1-related
invasion
migration
Journal
Journal of clinical laboratory analysis
ISSN: 1098-2825
Titre abrégé: J Clin Lab Anal
Pays: United States
ID NLM: 8801384
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
12
01
2020
revised:
01
05
2020
accepted:
14
05
2020
pubmed:
18
6
2020
medline:
15
7
2021
entrez:
18
6
2020
Statut:
ppublish
Résumé
Huntingtin-interacting protein 1-related (HIP1R) is a multi-domain gene that exerts many cellular functions including altering T cell-mediated cytotoxicity and controlling intracellular trafficking. However, its clinical significance and function in gastric cancer (GC) have not been described. The expression levels of HIP1R were tested by the transcriptional and translational expression analysis and immunohistochemistry (IHC) in matched adjacent non-tumorous vs tumor tissue specimens. The biological function of HIP1R on apoptosis, migration, and proliferation was evaluated by flow cytometry, Transwell, Cell Counting Kit-8 (CCK-8) assays, colony formation assays, and EdU labeling assays, respectively. We found downregulated HIP1R in GC compared with adjacent non-tumorous tissue, and HIP1R expression associated with N classification. We further found that the expression of HIP1R could induce apoptosis and inhibit proliferation, migration, invasion of GC cells, possibly through modulating Akt. Our data indicate that HIP1R may act as a potential diagnostic biomarker and a tumor suppressor gene in GC, potentially representing a novel therapeutic target for future GC treatment.
Sections du résumé
BACKGROUND
BACKGROUND
Huntingtin-interacting protein 1-related (HIP1R) is a multi-domain gene that exerts many cellular functions including altering T cell-mediated cytotoxicity and controlling intracellular trafficking. However, its clinical significance and function in gastric cancer (GC) have not been described.
METHODS
METHODS
The expression levels of HIP1R were tested by the transcriptional and translational expression analysis and immunohistochemistry (IHC) in matched adjacent non-tumorous vs tumor tissue specimens. The biological function of HIP1R on apoptosis, migration, and proliferation was evaluated by flow cytometry, Transwell, Cell Counting Kit-8 (CCK-8) assays, colony formation assays, and EdU labeling assays, respectively.
RESULTS
RESULTS
We found downregulated HIP1R in GC compared with adjacent non-tumorous tissue, and HIP1R expression associated with N classification. We further found that the expression of HIP1R could induce apoptosis and inhibit proliferation, migration, invasion of GC cells, possibly through modulating Akt.
CONCLUSIONS
CONCLUSIONS
Our data indicate that HIP1R may act as a potential diagnostic biomarker and a tumor suppressor gene in GC, potentially representing a novel therapeutic target for future GC treatment.
Identifiants
pubmed: 32548851
doi: 10.1002/jcla.23425
pmc: PMC7521271
doi:
Substances chimiques
Adaptor Proteins, Signal Transducing
0
Biomarkers, Tumor
0
HIP1R protein, human
0
Microfilament Proteins
0
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e23425Subventions
Organisme : China Postdoctoral Science Foundation
ID : 2018M640267
Organisme : China Postdoctoral Science Foundation
ID : 2018M640268
Organisme : Xingliao Talents Program in Liaoning Province
ID : XLYC1807164
Organisme : Project of Science and Technology of Shenyang
ID : 18-014-4-07
Organisme : National Natural Science Foundation of China
ID : 81872031
Organisme : National Natural Science Foundation of China
ID : U1908207
Informations de copyright
© 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.
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