Cross-Linked Self-Assembling Peptides and Their Post-Assembly Functionalization via One-Pot and In Situ Gelation System.
EDC
cross-linking
functional motifs
mechanical properties
rheology
self-assembling peptides
sulfo–NHS
supramolecular hydrogels
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
15 Jun 2020
15 Jun 2020
Historique:
received:
08
05
2020
revised:
11
06
2020
accepted:
12
06
2020
entrez:
19
6
2020
pubmed:
19
6
2020
medline:
18
2
2021
Statut:
epublish
Résumé
Supramolecular nanostructures formed through peptide self-assembly can have a wide range of applications in the biomedical landscape. However, they often lose biomechanical properties at low mechanical stress due to the non-covalent interactions working in the self-assembling process. Herein, we report the design of cross-linked self-assembling peptide hydrogels using a one-pot in situ gelation system, based on 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide/N-hydroxysulfosuccinimide (EDC/sulfo-NHS) coupling, to tune its biomechanics. EDC/sulfo-NHS coupling led to limited changes in storage modulus (from 0.9 to 2 kPa), but it significantly increased both the strain (from 6% to 60%) and failure stress (from 19 to 35 Pa) of peptide hydrogel without impairing the spontaneous formation of β-sheet-containing nano-filaments. Furthermore, EDC/sulfo-NHS cross-linking bestowed self-healing and thixotropic properties to the peptide hydrogel. Lastly, we demonstrated that this strategy can be used to incorporate bioactive functional motifs after self-assembly on pre-formed nanostructures by functionalizing an Ac-LDLKLDLKLDLK-CONH
Identifiants
pubmed: 32549405
pii: ijms21124261
doi: 10.3390/ijms21124261
pmc: PMC7353005
pii:
doi:
Substances chimiques
1-ethyl-3-(3-(diethylamino)propyl)carbodiimide
0
Carbodiimides
0
Hydrogels
0
Peptides
0
Succinimides
0
N-hydroxysulfosuccinimide
82436-78-0
Gelatin
9000-70-8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministero della Salute
ID : ricerca corrente 2018-2020
Références
Exp Cell Res. 2016 Apr 10;343(1):42-53
pubmed: 26524510
Acta Biomater. 2019 Jul 1;92:132-144
pubmed: 31075516
Anal Biochem. 1990 Feb 15;185(1):131-5
pubmed: 2344038
Nanoscale. 2013 Jul 21;5(14):6413-21
pubmed: 23739953
J Biomed Nanotechnol. 2014 Feb;10(2):309-23
pubmed: 24738339
PLoS One. 2007 Feb 07;2(2):e190
pubmed: 17285144
J Musculoskelet Neuronal Interact. 2007 Oct-Dec;7(4):335
pubmed: 18094500
J Appl Phys. 2010 Mar 15;107(6):63509
pubmed: 21464912
ACS Appl Mater Interfaces. 2018 Dec 5;10(48):41046-41055
pubmed: 30475573
Angew Chem Int Ed Engl. 2011 Jul 4;50(28):6292-5
pubmed: 21626619
Acta Biomater. 2018 Jan 15;66:258-271
pubmed: 29128535
Biomaterials. 2015 Jul;56:92-103
pubmed: 25934283
J Neurosci Res. 2010 Nov 1;88(14):3161-70
pubmed: 20818775
Methods Mol Biol. 2010;627:55-73
pubmed: 20217613
Biomaterials. 2005 Jun;26(16):3341-51
pubmed: 15603830
Nat Rev Mol Cell Biol. 2017 Dec;18(12):728-742
pubmed: 29115301
ACS Appl Mater Interfaces. 2016 Jan 27;8(3):2348-59
pubmed: 26720334
Faraday Discuss. 2013;166:381-97
pubmed: 24611289
Nat Rev Mater. 2016;1:
pubmed: 29214058
ACS Nano. 2015 Sep 22;9(9):9394-406
pubmed: 26284753
Biomater Sci. 2018 Dec 18;7(1):76-91
pubmed: 30475373
Bioact Mater. 2020 Jan 27;5(1):124-132
pubmed: 32128463
Adv Mater. 2018 May;30(20):e1705912
pubmed: 29602270
Biochim Biophys Acta. 2014 Aug;1840(8):2506-19
pubmed: 24418517
Nat Mater. 2014 Jun;13(6):645-52
pubmed: 24633344
PLoS One. 2011;6(5):e19782
pubmed: 21611127
Proc Natl Acad Sci U S A. 2019 Apr 9;116(15):7483-7492
pubmed: 30923117
Nat Mater. 2015 Dec;14(12):1269-77
pubmed: 26366848
Nat Mater. 2019 May;18(5):503-509
pubmed: 30988450
Nat Mater. 2016 Mar;15(3):326-34
pubmed: 26618884
J Huazhong Univ Sci Technolog Med Sci. 2009 Aug;29(4):512-6
pubmed: 19662373
Nanoscale. 2016 Jan 7;8(1):253-65
pubmed: 26607419
Science. 2016 Jan 29;351(6272):497-502
pubmed: 26823427
Adv Sci (Weinh). 2019 Jan 11;6(4):1801716
pubmed: 30828535
J Am Chem Soc. 2017 Jun 14;139(23):8044-8050
pubmed: 28581735
Interface Focus. 2017 Dec 6;7(6):20170028
pubmed: 29147558
Nanoscale. 2016 Oct 27;8(42):18105-18112
pubmed: 27734052
Proc Natl Acad Sci U S A. 2002 Jul 23;99(15):9996-10001
pubmed: 12119393
Macromol Biosci. 2007 May 10;7(5):544-51
pubmed: 17477441
Nanoscale. 2012 Apr 28;4(9):2946-57
pubmed: 22476090
ACS Biomater Sci Eng. 2017 Dec 11;3(12):3146-3160
pubmed: 29250593
Biophys J. 2009 Aug 19;97(4):1013-21
pubmed: 19686648
Langmuir. 2010 Mar 2;26(5):3641-7
pubmed: 19817454
Org Lett. 2019 Jul 19;21(14):5480-5484
pubmed: 31246483
Biomaterials. 2019 May;202:1-11
pubmed: 30818087
PLoS One. 2006 Dec 27;1:e119
pubmed: 17205123
Trends Biotechnol. 2017 Feb;35(2):145-158
pubmed: 27717599
Nat Mater. 2017 Dec;16(12):1233-1242
pubmed: 29115291
Soft Matter. 2016 Feb 14;12(6):1830-5
pubmed: 26738771
Front Neuroeng. 2010 Feb 08;3:1
pubmed: 20162033
Acta Biomater. 2019 Aug;94:351-360
pubmed: 31200117
J Mol Biol. 2009 Jan 30;385(4):1052-63
pubmed: 19038267
Biotechnol Bioeng. 2016 Feb;113(2):253-9
pubmed: 26134352
N Biotechnol. 2013 Jun 25;30(5):552-62
pubmed: 23541699