Protective Effects of Clostridium Butyricum in a Murine Model of Dextran Sodium Sulfate-Induced Colitis That Involve Inhibition of the TLR2 Signaling Pathway and T Helper 17 Cells.
Animals
Body Weight
Clostridium butyricum
Colitis
/ chemically induced
Dextran Sulfate
/ toxicity
Disease Models, Animal
Drinking Behavior
Feeding Behavior
Female
Gastrointestinal Microbiome
Inflammatory Bowel Diseases
/ immunology
Interleukin-17
/ genetics
Interleukin-23
/ genetics
Male
Mice
Mice, Inbred BALB C
Myeloid Differentiation Factor 88
/ genetics
Nuclear Receptor Subfamily 1, Group F, Member 3
/ genetics
Probiotics
RNA, Messenger
/ metabolism
Random Allocation
Signal Transduction
Th17 Cells
/ immunology
Toll-Like Receptor 2
/ genetics
Transcription Factor RelA
/ genetics
Clostridium butyricum
Dextran sodium sulfate
Inflammatory bowel disease
T helper 17 cells
Toll-like receptor2
Journal
The American journal of the medical sciences
ISSN: 1538-2990
Titre abrégé: Am J Med Sci
Pays: United States
ID NLM: 0370506
Informations de publication
Date de publication:
08 2020
08 2020
Historique:
received:
22
10
2019
revised:
08
03
2020
accepted:
14
05
2020
pubmed:
20
6
2020
medline:
6
10
2020
entrez:
20
6
2020
Statut:
ppublish
Résumé
This study aimed to investigate the role of Clostridium butyricum (C. butyricum) in conjunction with the Toll-like receptor2 (TLR2) signaling pathway and T helper 17 (Th17) cells in dextran sodium sulfate (DSS)-induced colitis in mice. Forty 8-week-old BALB/c mice were randomly divided into 5 groups of 8 mice for 7 days: control, DSS (5% DSS), DSS+C. butyricum (1 × 10 C. butyricum dose-dependently decreased DAI and histological damage scores in DSS mice and down-regulated the mRNA and protein levels of TLR2, MyD88 and NF-κBp65 in mouse colon tissue (all P < 0.05). In addition, C. butyricum dose-dependently decreased the levels of CD3 C. butyricum exerts a dose-dependently protective effect on acute intestinal inflammation induced by DSS in mice, by inhibiting the TLR2 signaling pathway, down-regulating the expression of IL23 and RORγt, and inhibiting the secretion of IL17.
Sections du résumé
BACKGROUND
This study aimed to investigate the role of Clostridium butyricum (C. butyricum) in conjunction with the Toll-like receptor2 (TLR2) signaling pathway and T helper 17 (Th17) cells in dextran sodium sulfate (DSS)-induced colitis in mice.
METHODS
Forty 8-week-old BALB/c mice were randomly divided into 5 groups of 8 mice for 7 days: control, DSS (5% DSS), DSS+C. butyricum (1 × 10
RESULTS
C. butyricum dose-dependently decreased DAI and histological damage scores in DSS mice and down-regulated the mRNA and protein levels of TLR2, MyD88 and NF-κBp65 in mouse colon tissue (all P < 0.05). In addition, C. butyricum dose-dependently decreased the levels of CD3
CONCLUSIONS
C. butyricum exerts a dose-dependently protective effect on acute intestinal inflammation induced by DSS in mice, by inhibiting the TLR2 signaling pathway, down-regulating the expression of IL23 and RORγt, and inhibiting the secretion of IL17.
Identifiants
pubmed: 32553747
pii: S0002-9629(20)30194-4
doi: 10.1016/j.amjms.2020.05.021
pii:
doi:
Substances chimiques
Il17a protein, mouse
0
Interleukin-17
0
Interleukin-23
0
Myd88 protein, mouse
0
Myeloid Differentiation Factor 88
0
Nuclear Receptor Subfamily 1, Group F, Member 3
0
RNA, Messenger
0
Rela protein, mouse
0
Rorc protein, mouse
0
Tlr2 protein, mouse
0
Toll-Like Receptor 2
0
Transcription Factor RelA
0
Dextran Sulfate
9042-14-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
176-191Informations de copyright
Copyright © 2020 Southern Society for Clinical Investigation. Published by Elsevier Inc. All rights reserved.