Plant SNAREs SYP22 and SYP23 interact with Tobacco mosaic virus 126 kDa protein and SYP2s are required for normal local virus accumulation and spread.

Arabidopsis / genetics Arabidopsis Proteins / genetics Gene Silencing Plant Diseases / genetics Protein Binding Qa-SNARE Proteins / genetics Nicotiana / genetics Tobacco Mosaic Virus / genetics Viral Proteins / genetics
Bimolecular fluorescence complementation Pre-vacuolar compartment Syntaxin Tobamoviruses Vacuole Vesicle trafficking Virus-host interaction Yeast two hybrid

Journal

Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674

Informations de publication

Date de publication:
08 2020
Historique:
received: 20 01 2020
revised: 18 03 2020
accepted: 07 04 2020
entrez: 21 6 2020
pubmed: 21 6 2020
medline: 7 2 2021
Statut: ppublish

Résumé

Viral proteins often interact with multiple host proteins during virus accumulation and spread. Identities and functions of all interacting host proteins are not known. Through a yeast two-hybrid screen an Arabidopsis thaliana Qa-SNARE protein [syntaxin of plants 23 (AtSYP23)], associated with pre-vacuolar compartment and vacuolar membrane fusion activities, interacted with Tobacco mosaic virus (TMV) 126 kDa protein, associated with virus accumulation and spread. In planta, AtSYP23 and AtSYP22 each fused with mCherry, co-localized with 126 kDa protein-GFP. Additionally, A. thaliana and Nicotiana benthamiana SYP2 proteins and 126 kDa protein interacted during bimolecular fluorescence complementation analysis. Decreased TMV accumulation in Arabidopsis plants lacking SYP23 and in N. benthamiana plants subjected to virus-induced gene silencing (VIGS) of SYP2 orthologs was observed. Diminished TMV accumulation during VIGS correlated with less intercellular virus spread. The inability to eliminate virus accumulation suggests that SYP2 proteins function redundantly for TMV accumulation, as for plant development.

Identifiants

DOI: 10.1016/j.virol.2020.04.002 PMID: 32560905
pubmed: 32560905
pii: S0042-6822(20)30069-6
doi: 10.1016/j.virol.2020.04.002
pii:
doi:

Substances chimiques

Arabidopsis Proteins 0
Qa-SNARE Proteins 0
Vam3 protein, Arabidopsis 0
Viral Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

57-71

Commentaires et corrections

Type : ErratumIn

Informations de copyright

Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.

Auteurs

Amr Ibrahim (A)

Noble Research Institute, LLC, Ardmore, OK, 73401, USA; Department of Nucleic Acid and Protein Structure, Agricultural Genetic Engineering Research Institute, Agricultural Research Center, Giza, Egypt. Electronic address: ayibrahim@noble.org.

Xiaohua Yang (X)

Noble Research Institute, LLC, Ardmore, OK, 73401, USA.

Chengke Liu (C)

Noble Research Institute, LLC, Ardmore, OK, 73401, USA.

Kimberly D Cooper (KD)

Noble Research Institute, LLC, Ardmore, OK, 73401, USA.

Bethany A Bishop (BA)

Noble Research Institute, LLC, Ardmore, OK, 73401, USA.

Min Zhu (M)

Noble Research Institute, LLC, Ardmore, OK, 73401, USA.

Soonil Kwon (S)

Noble Research Institute, LLC, Ardmore, OK, 73401, USA.

James E Schoelz (JE)

Division of Plant Sciences, University of Missouri, Columbia, MO, 65211, USA.

Richard S Nelson (RS)

Noble Research Institute, LLC, Ardmore, OK, 73401, USA. Electronic address: rnelso@okstate.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › Plant SNAREs SYP22 and SYP23 interact with...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Protéines d'Arabidopsis › Plant SNAREs SYP22 and SYP23 interact with...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Plant SNAREs SYP22 and SYP23 interact with...
questionsmedicales.fr › Phénomènes physiologiques des plantes › Maladies des plantes › Plant SNAREs SYP22 and SYP23 interact with...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Plant SNAREs SYP22 and SYP23 interact with...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines du transport vésiculaire › Protéines SNARE › Protéines Q-SNARE › Protéines Qa-SNARE › Plant SNAREs SYP22 and SYP23 interact with...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Tobamovirus › Virus de la mosaïque du tabac › Plant SNAREs SYP22 and SYP23 interact with...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Plant SNAREs SYP22 and SYP23 interact with...