Livestock Gene Editing by One-step Embryo Manipulation.

Animals CRISPR-Associated Protein 9 CRISPR-Cas Systems / genetics Clustered Regularly Interspaced Short Palindromic Repeats Gene Editing / veterinary Livestock
Animal model CRISPR/Cas9 Embryo Gene editing Livestock

Journal

Journal of equine veterinary science
ISSN: 0737-0806
Titre abrégé: J Equine Vet Sci
Pays: United States
ID NLM: 8216840

Informations de publication

Date de publication:
06 2020
Historique:
received: 18 02 2020
revised: 06 04 2020
accepted: 07 04 2020
entrez: 22 6 2020
pubmed: 22 6 2020
medline: 23 1 2021
Statut: ppublish

Résumé

The breakthrough and rapid advance of clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) technology has enabled the efficient generation of gene-edited animals by one-step embryo manipulation. Clustered regularly interspaced short palindromic repeat/CRISPR-associated protein 9 delivery to the livestock embryos has been typically achieved by intracytoplasmic microinjection; however, recent studies show that electroporation may be a reliable, efficient, and practical method for CRISPR/Cas9 delivery. The source of embryos used to generate gene-edited animals varies from in vivo to in vitro produced, depending mostly on the species of interest. In addition, different Cas9 and gRNA reagents can be used for embryo editing, ranging from Cas9-coding plasmid or messenger RNA to Cas9 recombinant protein, which can be combined with in vitro transcribed or synthetic guide RNAs. Mosaicism is reported as one of the main problems with generation of animals by embryo editing. On the other hand, off-target mutations are rarely found in livestock derived from one-step editing. In this review, we discussed these and other aspects of generating gene-edited animals by single-step embryo manipulation.

Identifiants

DOI: 10.1016/j.jevs.2020.103025 PMID: 32563448
pubmed: 32563448
pii: S0737-0806(20)30116-7
doi: 10.1016/j.jevs.2020.103025
pii:
doi:

Substances chimiques

CRISPR-Associated Protein 9 EC 3.1.-

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S. Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

103025

Informations de copyright

Copyright © 2020 Elsevier Inc. All rights reserved.

Auteurs

Sergio Navarro-Serna (S)

Department of Animal Science, University of California, Davis, CA; Department of Physiology, International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), University of Murcia, Murcia, Spain; Institute for Biomedical Research of Murcia IMIB-Arrixaca, Murcia, Spain.

Marcela Vilarino (M)

Department of Animal Science, University of California, Davis, CA.

Insung Park (I)

Department of Animal Science, University of California, Davis, CA.

Joaquin Gadea (J)

Department of Physiology, International Excellence Campus for Higher Education and Research (Campus Mare Nostrum), University of Murcia, Murcia, Spain; Institute for Biomedical Research of Murcia IMIB-Arrixaca, Murcia, Spain.

Pablo Juan Ross (PJ)

Department of Animal Science, University of California, Davis, CA. Electronic address: Pross@ucdavis.edu.

Articles similaires

Evaluating the efficacy of telesurgery with dual console SSI Mantra Surgical Robotic System: experiment on animal model and clinical trials.

Sudhir Prem Srivastava, Vishwajyoti Pascual Srivastava, Avinesh Singh et al.
1.00
Robotic Surgical Procedures Animals Humans Telemedicine Models, Animal

Odour generalisation and detection dog training.

Lyn Caldicott, Thomas W Pike, Helen E Zulch et al.
1.00
Animals Odorants Dogs Generalization, Psychological Smell

FBXO22 inhibits colitis and colorectal carcinogenesis by regulating the degradation of the S2448-phosphorylated form of mTOR.

Minle Li, Xuan Chen, Pengfei Qu et al.
1.00
Animals TOR Serine-Threonine Kinases Colorectal Neoplasms Colitis Mice

Use of organic material provided by an automatic enrichment device by weaner pigs and its influence on tail lesions.

Philipp Heseker, Jeanette Probst, Stefanie Ammer et al.
1.00
Animals Tail Swine Behavior, Animal Animal Husbandry

Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Livestock Gene Editing by One-step Embryo Manipulation.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines associées aux CRISPR › Protéine-9 associée à CRISPR › Livestock Gene Editing by One-step Embryo Manipulation.
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › Livestock Gene Editing by One-step Embryo Manipulation.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › Livestock Gene Editing by One-step Embryo Manipulation.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Édition de gène › Livestock Gene Editing by One-step Embryo Manipulation.
questionsmedicales.fr › Eucaryotes › Animaux › Groupes animaux › Animaux domestiques › Bétail › Livestock Gene Editing by One-step Embryo Manipulation.