The MAIT TCRβ chain contributes to discrimination of microbial ligand.
Antimicrobial responses
T-cell receptor
bacterial host response
Journal
Immunology and cell biology
ISSN: 1440-1711
Titre abrégé: Immunol Cell Biol
Pays: United States
ID NLM: 8706300
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
06
05
2019
revised:
03
11
2019
revised:
10
05
2020
revised:
18
06
2020
accepted:
19
06
2020
pubmed:
23
6
2020
medline:
25
8
2021
entrez:
23
6
2020
Statut:
ppublish
Résumé
Mucosal-associated invariant T (MAIT) cells are key players in the immune response against microbial infection. The MAIT T-cell receptor (TCR) recognizes a diverse array of microbial ligands, and recent reports have highlighted the variability in the MAIT TCR that could further contribute to discrimination of ligand. The MAIT TCR complementarity determining region (CDR)3β sequence displays a high level of diversity across individuals, and clonotype usage appears to be dependent on antigenic exposure. To address the relationship between the MAIT TCR and microbial ligand, we utilized a previously defined panel of MAIT cell clones that demonstrated variability in responses against different microbial infections. Sequencing of these clones revealed four pairs, each with shared (identical) CDR3α and different CDR3β sequences. These pairs demonstrated varied responses against microbially infected dendritic cells as well as against 5-(2-oxopropylideneamino)-6-d-ribitylaminouracil, a ligand abundant in Salmonella enterica serovar Typhimurium, suggesting that the CDR3β contributes to differences in ligand discrimination. Taken together, these results highlight a key role for the MAIT CDR3β region in distinguishing between MR1-bound antigens and ligands.
Identifiants
pubmed: 32568415
doi: 10.1111/imcb.12370
pmc: PMC7541710
mid: NIHMS1620115
doi:
Substances chimiques
5-(2-oxopropylideneamino)-6-d-ribitylaminouracil
0
Complementarity Determining Regions
0
Ligands
0
Ribitol
488-81-3
Uracil
56HH86ZVCT
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
770-781Subventions
Organisme : NIAID NIH HHS
ID : R01 AI048090
Pays : United States
Organisme : NIAID NIH HHS
ID : K08 AI118538
Pays : United States
Organisme : BLRD VA
ID : I01 BX000533
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL083808
Pays : United States
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 100326/Z/12/Z
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : R01 AI147954
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI134790
Pays : United States
Informations de copyright
© 2020 Australian and New Zealand Society for Immunology Inc.
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