Discovery of Turn-On Fluorescent Probes for Detecting PDEδ Protein in Living Cells and Tumor Slices.
Animals
Biomarkers
/ metabolism
Catalytic Domain
Cell Survival
/ drug effects
Fluorescent Dyes
/ chemistry
Gene Expression Regulation, Enzymologic
/ physiology
Gene Expression Regulation, Neoplastic
/ physiology
Humans
MAP Kinase Kinase 7
/ genetics
Mice
Mitogen-Activated Protein Kinase 1
/ genetics
Molecular Structure
Neoplasms
/ enzymology
Phosphoric Diester Hydrolases
/ chemistry
Protein Conformation
Proto-Oncogene Proteins c-akt
/ genetics
Proto-Oncogene Proteins c-raf
/ genetics
Proto-Oncogene Proteins p21(ras)
/ genetics
Skin
/ enzymology
TOR Serine-Threonine Kinases
/ genetics
Journal
Analytical chemistry
ISSN: 1520-6882
Titre abrégé: Anal Chem
Pays: United States
ID NLM: 0370536
Informations de publication
Date de publication:
21 07 2020
21 07 2020
Historique:
pubmed:
24
6
2020
medline:
24
2
2021
entrez:
24
6
2020
Statut:
ppublish
Résumé
The first small-molecule fluorescent turn-on probes for detecting PDEδ protein were rationally designed, showing reasonable fluorescent properties and the fluorescent ability has been applied for visualization of the PDEδ protein in living cells and at tissue levels. The qPCR results showed that the mRNA expression of
Identifiants
pubmed: 32571022
doi: 10.1021/acs.analchem.0c00335
doi:
Substances chimiques
Biomarkers
0
Fluorescent Dyes
0
KRAS protein, human
0
MTOR protein, human
EC 2.7.1.1
AKT1 protein, human
EC 2.7.11.1
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Proto-Oncogene Proteins c-raf
EC 2.7.11.1
Raf1 protein, human
EC 2.7.11.1
TOR Serine-Threonine Kinases
EC 2.7.11.1
MAPK1 protein, human
EC 2.7.11.24
Mitogen-Activated Protein Kinase 1
EC 2.7.11.24
MAP Kinase Kinase 7
EC 2.7.12.2
MAP2K7 protein, human
EC 2.7.12.2
Phosphoric Diester Hydrolases
EC 3.1.4.-
Proto-Oncogene Proteins p21(ras)
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM