Proteome Analysis Reveals Syndecan 1 Regulates Porcine Sapelovirus Replication.
quantitative proteomics
replication
sapelovirus
tandem mass tag
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
19 Jun 2020
19 Jun 2020
Historique:
received:
27
03
2020
revised:
03
06
2020
accepted:
11
06
2020
entrez:
25
6
2020
pubmed:
25
6
2020
medline:
13
2
2021
Statut:
epublish
Résumé
Porcine sapelovirus A (PSV) is a single stranded, positive-sense, non-enveloped RNA virus that causes enteritis, pneumonia, polioencephalomyelitis, and reproductive disorders in pigs. Research on PSV infection and interaction with host cells is unclear. In this study, we applied tandem mass tag proteomics analysis to investigate the differentially expressed proteins (DEPs) in PSV-infected pig kidney (PK)-15 cells and explored the interactions between PSV and host cells. Here we mapped 181 DEPs, including 59 up-regulated and 122 down-regulated DEPs. Among them, osteopontin (SPP1), induced protein with tetratricopeptide repeats 5 (IFIT5), ISG15 ubiquitin-like modifier (ISG15), vinculin (VCL), and syndecan-1 (SDC1) were verified significantly changed using RT-qPCR. Additionally, overexpression of SDC1 promoted PSV viral protein (VP)1 synthesis and virus titer, and silencing of SDC1 revealed the opposite results. Our findings show that SDC1 is a novel host protein and plays crucial roles in regulating PSV replication.
Identifiants
pubmed: 32575635
pii: ijms21124386
doi: 10.3390/ijms21124386
pmc: PMC7352226
pii:
doi:
Substances chimiques
Syndecan-1
0
Viral Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : National Basic Research Program of China (973 Program)
ID : No.2017YFC1200203
Organisme : National Natural Science Foundation of China
ID : 31572525
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