Inhibitory effect of melatonin on hypoxia-induced vasculogenic mimicry via suppressing epithelial-mesenchymal transition (EMT) in breast cancer stem cells.
Angiogenic Proteins
/ genetics
Antineoplastic Agents
/ pharmacology
Breast Neoplasms
/ drug therapy
Cell Movement
/ drug effects
Cell Proliferation
/ drug effects
Drug Resistance, Neoplasm
Epithelial-Mesenchymal Transition
/ drug effects
Female
Gene Expression Regulation, Neoplastic
Humans
Hypoxia-Inducible Factor 1, alpha Subunit
/ genetics
MCF-7 Cells
Melatonin
/ pharmacology
Molecular Mimicry
Neoplasm Invasiveness
Neoplastic Stem Cells
/ drug effects
Neovascularization, Pathologic
Phenotype
Signal Transduction
Tumor Hypoxia
Tumor Microenvironment
Breast cancer
Cancer stem cell
Hypoxia
Melatonin
Vasculogenic mimicry
Journal
European journal of pharmacology
ISSN: 1879-0712
Titre abrégé: Eur J Pharmacol
Pays: Netherlands
ID NLM: 1254354
Informations de publication
Date de publication:
15 Aug 2020
15 Aug 2020
Historique:
received:
20
02
2020
revised:
09
06
2020
accepted:
15
06
2020
pubmed:
25
6
2020
medline:
11
5
2021
entrez:
25
6
2020
Statut:
ppublish
Résumé
Vasculogenic mimicry (VM) play an important role in breast cancer metastasis and anti- angiogenic drugs resistance. Hypoxia, the epithelial-mesenchymal transition (EMT), and cancer stem cells (CSCs) are known as essential factors for VM formation. Also, melatonin is an amino acid-derived hormone with many anti-tumor effects. Despite the antitumor effects of melatonin, its effect on VM formation in breast cancer has not been considered yet, so we investigated the effect of melatonin on VM formation through EMT process under hypoxia conditions in breast CSCs. The CSCs percentage and VM formation were determined in MCF-7 and MDA-MB-231, respectively. Also, analysis of HIF-1α expression under hypoxia in MDA-MB-231 and MCF-7 cell lines was performed using Western blot. The effect of melatonin on the VM formation, invasion, and migration was also investigated. Moreover, the effect of melatonin on the expression EMT markers was evaluated. CD44
Identifiants
pubmed: 32580038
pii: S0014-2999(20)30374-5
doi: 10.1016/j.ejphar.2020.173282
pii:
doi:
Substances chimiques
Angiogenic Proteins
0
Antineoplastic Agents
0
HIF1A protein, human
0
Hypoxia-Inducible Factor 1, alpha Subunit
0
Melatonin
JL5DK93RCL
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
173282Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.