Functional Heterogeneity of Protein Kinase A Activation in Multipotent Stromal Cells.
PKA-Spark biosensor
functional heterogeneity
intracellular signaling
mesenchymal stem/stromal cells
multipotent stromal cells
protein kinase A
single cell analysis
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
22 Jun 2020
22 Jun 2020
Historique:
received:
30
05
2020
revised:
17
06
2020
accepted:
17
06
2020
entrez:
26
6
2020
pubmed:
26
6
2020
medline:
20
3
2021
Statut:
epublish
Résumé
Multipotent stromal cells (MSC) demonstrate remarkable functional heterogeneity; however, its molecular mechanisms remain largely obscure. In this study, we explored MSC response to hormones, which activate Gs-protein / cyclic AMP (cAMP) / protein kinase A (PKA) dependent signaling, at the single cell level using genetically encoded biosensor PKA-Spark. For the first time, we demonstrated that about half of cultured MSCs are not able to activate the cAMP/PKA pathway, possibly due to the limited availability of adenylyl cyclases. Using this approach, we showed that MSC subpopulations responding to various hormones largely overlapped, and the share of responding cells did not exceed 40%. Using clonal analysis, we showed that signaling heterogeneity of MSC could be formed de novo within 2 weeks.
Identifiants
pubmed: 32580466
pii: ijms21124442
doi: 10.3390/ijms21124442
pmc: PMC7353043
pii:
doi:
Substances chimiques
Hormones
0
Cyclic AMP
E0399OZS9N
Cyclic AMP-Dependent Protein Kinases
EC 2.7.11.11
Adenylyl Cyclases
EC 4.6.1.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Russian Science Foundation
ID : 19-75-30007
Organisme : Russian Foundation for Basic Research
ID : 19-315-80018
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